乙型肝炎患者外周血淋巴细胞表达的变化  

Changes in expression of T-lymphocyte subsets of peripheral blood in patients with chronic hepatitis B

作　　者：张平[1] 袁迎春[1] 张爱芸[1] 张栩[1] 李玉芳[1] 丁向春[1] 

机构地区：[1]宁夏医科大学附属医院感染病科,银川750004

出　　处：《江苏医药》2010年第6期625-627,共3页Jiangsu Medical Journal

基　　金：宁夏自然科学基金项目(NZ0794)

摘　　要：目的分析乙型肝炎患者外周血淋巴细胞亚群的表达。方法对112例乙型肝炎患者采用荧光定量PCR技术和流式细胞术分别检测血中HBVDNA及外周血淋巴细胞亚群CD3+T细胞、CD4+T细胞、CD8+T细胞、CD3-/CD16+CD56+(NK细胞)、CD3-CD19+(B细胞)表达百分比,进行各淋巴细胞亚群百分比统计学分析。结果与急性乙型肝炎(AHB)组比较,慢性乙型肝炎(CHB)组、肝炎肝硬化(LC)组CD3+T细胞、CD4+T细胞、CD8+T细胞、CD4+/CD8+比值均有显著性下降,LC组下降最显著(P<0.01)。而LC组B细胞百分比较CHB、AHB组高(P<0.01)。NK细胞百分比较CHB、AHB组均存在降低趋势(P<0.01)。结论乙型肝炎患者体内存在T细胞亚群失衡和细胞免疫功能紊乱;外周血T细胞亚群随病情进展而减少。Objective To study the expressions of T cells subsets in peripheral blood in hepatitis B virus（HBV） patients.Methods Using PCR and flow cytometry,HBV DNA and T cell subsets of CD3＋,CD4＋,CD8＋,CD3-/CD16＋CD56＋,CD3-CD19＋ cells in peripheral blood of 112 HBV cases were examined and statistically analyzed.Results Compared to the patients with acute hepatitis,in the patients with chronic hepatitis and liver sclerosis,the percentages of CD3＋,CD4＋,CD8＋,CD4＋/CD8＋ were significantly reduced,particularly in sclerosis patients（P0.01）.The percentage of B cells in sclerosis patients was significantly higher than that in the patients with chronic and acute hepatitis.NK cells in sclerosis patients tended to decrease（P0.01）.Conclusion There is an imbalance of T cell subsets and disturbance of cell immune function.T cell subsets are decreasing as the disease is deteriorating in HBV patients.

关 键 词：乙型肝炎 淋巴细胞亚群 

分 类 号：R512[医药卫生—内科学]