多孔载因子CPC促进骨缺损愈合的实验研究  

Experimental investigations of macroporous calcium phosphate cements/bone morphogenic protein composite in repairing bone defects

作　　者：余雷[1] 刘兴炎[2] 厉孟[2] 葛宝丰[2] 梁建平[2] 

机构地区：[1]第四军医大学西京医院,西安710032 [2]兰州军区总医院,兰州730050

出　　处：《中国矫形外科杂志》2010年第8期673-677,共5页Orthopedic Journal of China

基　　金：全军"十一五"医药卫生科研基金资助项目(编号:06MA090);甘肃省自然科学基金资助项目(编号:0710RJZA068)

摘　　要：[目的]探讨磷酸钙骨水泥复合rhBMP-2/明胶微球复合材料在治疗骨缺损时的降解、成骨性能。[方法]制备携载rhBMP-2的明胶微球(GMs),与磷酸钙骨水泥(CPC)复合,制备出rhBMP-2/GMs/CPC复合人工骨。取30只新西兰大白兔,在前臂桡骨中段制造人工骨缺损,随机分成3组,分别植入rhBMP-2/GMs/CPC/复合物(A组)、GMs/CPC(B组)、rhBMP-2/CPC(C组),术后6、12周分别进行X线检测、骨密度测定,术后12周处死动物,分别行生物力学测定,脱钙切片、HE染色,不脱钙切片进行荧光显微镜下观察双标间距,计算平均矿化率。[结果]与GMs/CPC、rhBMP-2/CPC组比较,复合材料植入后不同时间点的材料降解及成骨均高于对照组。12周A组标本生物力学实验测定结果表明指标接近正常,与B、C组比较有统计学差异。骨密度12周、新骨矿化率提示有统计学差异。[结论]rhBMP-2/GMs/CPC微球系统复合材料在体内易降解,具有良好成骨活性,是良好的骨修复材料。[ Objective] To analyze the biodegradation properties and active osteogenesis features of implanted composites of gelatin microspheres （GMs） impregnated with calcium phosphate cement （CPC） and recombinant human bone morphogenetic protein- 2 （rhBMP- 2） in bone defect. [ Method] rhBMP- 2 loaded gelatin microspheres was incorporated in rhBMP- 2/CPC during setting. Thirty rabbits were divided into groups A, B and C（ 10 each）. The rhBMP -2/GMs/CPC, GMs/cpc and rhBMP - 2/cpc was implanted into the rabbits of group A, B and C, respectively. The biodegradation properties and active osteogenesis features of implanted composites bone substitute was evaluated by radiography,histology, bone mineral density （BMD）after the composites were implanted into the bone defects at rabbit radius for 6,12 weeks respectively. [ Result] Significant implant resorption and bone ingrowth were observed in all rhBMP -2/GM/CPC treated defects while the defects treated with rhBMP -2/CPC implants showed slight resorption of CPC and the defects treated with GM/CPC implants showed slight formation of new bone. [ Conclusion] The result of this study suggested that the rhBMP -2/GMs/CPC composite could be used as material for bone graft substitute.

关 键 词：磷酸钙骨水泥 明胶微球 重组人骨形成蛋白-2 降解 骨替代 骨缺损 

分 类 号：R318.08[医药卫生—生物医学工程]