CXCR4在“人源性”乳腺癌骨转移小鼠模型中的表达及其意义  

Expression and significance of CXCR4 in a mouse model of human breast cancer metastasis to implanted human bone

作　　者：沈恩超[1] 王水[2] 凌立君[2] 黄中晶[1] 钱超[1] 许立生[1] 

机构地区：[1]江苏大学附属医院乳腺外科,镇江212001 [2]南京医科大学第一附属医院乳腺外科

出　　处：《江苏医药》2010年第7期813-816,共4页Jiangsu Medical Journal

摘　　要：目的探讨趋化因子受体CXCR4在"人源性"乳腺癌骨转移小鼠模型转移骨中的表达对判断乳腺癌预后的价值。方法建立"人源性"乳腺癌骨转移小鼠模型,SCID雌性小鼠50只随机分为实验组(n=30)与对照组(n=20)。实验组小鼠背部皮下植入人骨,4周后将移植骨成活鼠随机均分A,B,C亚组,分别注射MD-MBA-231干细胞亚群1×105个/只,1×106个/只及MD-MBA-231人乳腺癌亲代细胞1×106个/只。D组鼠(阳性对照组,未植入人骨)注射MD-MBA-231人乳腺癌亲代细胞1×106个/只;E组鼠(阴性对照组,植入人骨)注射生理盐水。第8周处死所有小鼠,取人骨、鼠骨组织。用免疫组化及实时定量PCR法检测其中CXCR4的表达,并进行定量和相关性分析。结果在小鼠模型中B组骨转移率最高(77.8%,P<0.05)。B组中人骨转移灶CXCR4抗原表达高于C、D组骨中的表达(P<0.05);B组CXCR4mRNA表达水平是C组8.4倍、D组28.4倍。结论CXCR4的表达与乳腺癌发生、转移密切相关;推测其可作为判断乳腺癌患者一个有价值的预后指标。Objective To investigate the expression and prognostic value of CXCR4 in mouse model of human breast cancer metastasis to implanted human bone.Methods Fifty SCID/berg female mice were randomly divided into experimental（n=30） and control group（n=20）.Four weeks after the model establishment,the experimental group had human bone implanted into dorsal flanks was divided into subgroups of A,B,C,which were injected 1×10^5,1×10^6 MD-MBA-231 human breast cancer stem-like cells,and 1×10^6 MD-MBA-231 parental cells,respectively.A positive control group D without implantation of human bone was also injected 1×10^6 MD-MBA-231 parental cells.A negative control group E with implantation of human bone was injected isotonic sodium chloride.All animals were sacrificed on the 8th week.CXCR4 expression was detected using immunohistochemistry and realtime-PCR assay.Results Bone metastatic morbidity（77.8%） in group B was the highest among all groups（P〈0.05）.The expression of CXCR4 in bone metastasis tissues was stronger in group B than that in groups of C and D（P〈0.05）.The mRNA level of CXCR4 in bone metastasis tissues of group B was 8.4 times as that in group C or 28.4 times as that in group D.ConclusionExpression of CXCR4 is closely related with tumor growth and metastasis of breast cancer,and could be taken as a valuable marker for evaluating the prognosis of the patients with breast cancer.

关 键 词：乳腺癌 CXCR4 骨转移小鼠模型 

分 类 号：R655[医药卫生—外科学]