隐藏嗜酸菌DX1－1和氧化亚铁硫杆菌CMS的紫外诱变育种及浸矿研究  

UV-induced mutagenesis and bioleaching of Acidiphilium cryptum and Acidithiobacillus ferrooxidans

作　　者：杨宇[1] 张帅[1] 徐爱玲[1] 邹俐宏[1] 历丽[1] 邱冠周[1] 

机构地区：[1]中南大学资源加工与生物工程学院,生物冶金教育部重点实验室,湖南长沙410083

出　　处：《中南大学学报（自然科学版）》2010年第2期393-399,共7页Journal of Central South University:Science and Technology

基　　金：国家重点基础研究发展计划(“973”计划)项目(2004CB619201);国家创新研究群体自然科学基金资助项目(50321402)

摘　　要：对从江西大兴黄铜矿的酸性矿坑水中分离得到的氧化亚铁硫杆菌CMS和隐藏嗜酸菌DX1-1进行紫外诱变育种及浸矿研究。结果表明:细菌CMS和DX1-1的最适生长温度为30℃,最适pH值分别为2.0和3.5;通过紫外诱变获得突变型隐藏嗜酸菌DX1-1和氧化亚铁硫杆菌CMS,最佳处理时间为60s,正突变率分别可达到16.7%和20.0%;诱变后的隐藏嗜酸菌DX1-1达到稳定期的时间比诱变前缩短20h,并且具有更大的菌体浓度;诱变后的氧化亚铁硫杆菌CMS氧化全部亚铁所需时间为48h,比诱变前菌株缩短11h;诱变后混合菌浸矿中,用原子吸收光谱法测定浸出30d后铜离子质量浓度达到2.78g/L,而紫外诱变前菌株浸出铜离子质量浓度为2.48g/L;生物浸出30d后,隐藏嗜酸菌DX1-1与氧化亚铁硫杆菌CMS的菌落个数比由1-1变为1-20左右。The original strains Acidithiobacillusferrooxidans CMS and Acidiphilium cryptum DX1-1 isolated from the drainage of some caves rich in chalcopyrite in Dexing in Jiangxi Province of China were studied by UV-induced mutagenesis and bioleaching. The results show that the optimum temperature and pH value are 30 ℃ and 3.5 for Ac. cryptum DX1-1, and 30 ℃ and 2.0 for At. ferrooxidans CMS, respectively. After being treated by UV radiating, the optimum UV radiating time of DX1-1 and CMS is 60 s and their positive mutation rates are 16.7% and 20.0%. Ac. cryptum after mutagenesis reaches stationary phase 20 h ahead of the original strain. The most active UV-mutated strain At. ferrooxidans CMS oxidizes all the ferrous ion in 48 h, which is 11 h less than its original strain. After bioleaching with the mixture of UV-mutated strains of At. ferrooxidans CMS and A c. cryptum DX1-1, 2.78 g/L of copper can be extracted, which can be measured by atomic absorption spectrometry after 30 d, while 2.48 g/L copper can be extracted with the mixture of the original strains before UV-mutation. After bioleaching for 30 d, the proportion of cell density in the cultures ofAc. cryptum DX 1-1 and At. ferrooxidans CMS is changed from 1：1 to approximately 1：20.

关 键 词：隐藏嗜酸菌 氧化亚铁硫杆菌 紫外诱变 生物浸出 

分 类 号：Q939.97[生物学—微生物学]