Tyr/Glu/Fe_3O_4/Nafion/CNT/GCE酪氨酸酶生物传感器的制备及应用于农药检测的研究  

Construction of Tyr/Glu/Fe_3O_4/Nafion/CNT/GCE Biosensor for Detection of Pesticide

作　　者：曲云鹤[1] 肖飞[1] 程欲晓[1] 施国跃[1] 金利通[1] 

机构地区：[1]华东师范大学化学系,上海200062

出　　处：《化学学报》2010年第6期535-539,共5页Acta Chimica Sinica

基　　金：上海世博专项(No.06dz05824)资助项目

摘　　要：制备了磁性四氧化三铁纳米粒子,采用碳纳米管、Nafion等功能性材料构建了酪氨酸酶(Tyr)生物传感器,并将其应用于农药的检测.实验表明,四氧化三铁纳米粒子具有良好的生物兼容性,碳纳米管能够有效地促进电子传递,修饰了四氧化三铁纳米粒子等功能性材料的酶传感器,响应速度快,检测灵敏度高,稳定性好;固定在传感器上的酪氨酸酶有良好的酶动力学响应,其表观米氏常数为61.5μmol/L.利用农药对酪氨酸酶的抑制作用,以苯酚为底物,对农药呋喃丹进行了检测,检测限达到2.0×10-9mol/L.A Tyr/Glu/Fe3O4/Nafion/CNT multilayer modified electrode was employed for the detection of carbofuran in samples. The configuration of the nanostructure on the electrode provides a favorable environment for the Tyr electrocatalytic characterization and fast electron transfer. The surface of the sensor was characterized by TEM methods. A set of experimental conditions were also optimized for the detection of the pesticide. The developed biosensor showed high sensitivity, stability and reproducibility, which had a low detection limit of 2.0×10^-9 mol/L for carbofuran.

关 键 词：四氧化三铁 酪氨酸酶 农药 呋喃丹 

分 类 号：TQ450.1[化学工程—农药化工]