三仁汤对脾胃湿热证大鼠血清醛固酮、血浆抗利尿激素影响的研究  

Effect of Sanren Decoction on Serum Aldosterone and Plasma Antidiuretic Hormone in Rats with Spleen-stomach Damp-heat Syndrome

作　　者：文小敏[1] 李云鹏[1] 武凯歌[1] 洪冰[1] 任玺[1] 林培政[2] 

机构地区：[1]南方医科大学中医药学院,广东广州510515 [2]广州中医药大学,广东广州510405

出　　处：《新中医》2010年第5期98-99,共2页New Chinese Medicine

基　　金：国家自然科学基金-广东联合基金(编号:U0632009);国家中医药管理局中医药科学技术研究专项(编号:06-07JP33);广东省中医药局科研基金(编号:2008195)

摘　　要：目的:观察脾胃湿热证大鼠醛固酮(ALD)和抗利尿激素(ADH)改变情况,探讨三仁汤的作用机理。方法:将50只雄性Wistar大鼠随机分为正常组、模型组、三仁汤(高、中、低剂量)组,通过复合因素(外部湿热环境加肥甘饮食加病原微生物)造模,采用放射免疫法检测大鼠血清ALD和血浆ADH的水平。结果:模型组大鼠血清ALD及血浆ADH升高明显,三仁汤(高、中、低剂量)组可以降低血清ALD和血浆ADH水平(P<0.05,P<0.01)。结论:醛固酮、抗利尿激素升高,可能是脾胃湿热证发生的机理之一;三仁汤调节ALD、ADH的释放水平,可能是其清热祛湿的作用机制之一。Objective:To investigate the therapeutic mechanism of Sanren Decoction.Methods:Fifty male Wistar rats were randomized into 5 groups:normal group,model group,and high-,middle-and low-dose SD groups.A combined method (the combination of damp-heat environment,fatty sweet diet and pathogens) was used for the establishment of spleen-stomach damp-heat rat models.Radioimmunoassay was applied in detecting serum aldosterone (ALD) and plasma antidiuretic hormone (ADH) levels.Results:Serum ALD and plasma ADH were much increased in the model group,and Sanren Decoction had an effect on decreasing the above indexes (P < 0.05 or P < 0.01)),Conclusion:The increase of serum ALD and plasma ADH is probably contributed to one of the pathogenesis of spleen-stomach damp-heat syndrome.Regulation of ALD and ADH levels is probably one if the mechanisms of Sanren Decoction in clearing heat and removing damp.

关 键 词：三仁汤 脾胃湿热证 醛固酮 抗利尿激素 动物实验 大鼠 

分 类 号：R285.5[医药卫生—中药学]