冠心病血瘀证血浆ApoA-Ⅰ和α_1-AT与炎症关系的研究  

Relationship between plasma ApoA-Ⅰ,α_1-AT and inflammation in blood stasis syndrome of coronary heart disease

作　　者：解华[1] 王伟[1] 赵慧辉[1] 郭淑贞[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《北京中医药大学学报》2010年第4期253-256,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)资助项目(No.2003CB517105);国家科技重大专项资助项目(No.2009ZX09502-018)

摘　　要：目的研究载脂蛋白A-Ⅰ(ApoA-Ⅰ)和α1-抗胰蛋白酶(α1-AT)在冠心病不稳定性心绞痛血瘀证患者血浆中的表达差异,探讨ApoA-Ⅰ和α1-AT与炎症的关系。方法严格按照冠心病不稳定性心绞痛血瘀证诊断标准,筛选得到气虚血瘀证、痰瘀互阻证患者的血浆样品34例,均于入院2d内取清晨空腹肘静脉血,EDTA抗凝,离心后采用Western印迹法(Western-blot)和酶联免疫法(ELISA)检测血浆ApoA-Ⅰ和α1-AT蛋白的表达水平。结果气虚血瘀证和痰瘀互阻证患者的ApoAⅠ-蛋白表达水平明显低于健康对照组(P<0.05);气虚血瘀证和痰瘀互阻证患者的α1-AT蛋白表达水平明显高于健康对照组(P<0.05)。结论冠心病不稳定性心绞痛血瘀证患者存在ApoA-Ⅰ和α1-AT蛋白的表达差异,认为炎症反应是冠心病血瘀证形成的机制之一。Objective To study the expression difference of plasma apolipoprotein A-Ⅰ （ApoA-Ⅰ） and α1-antitrypsin （α1-AT） in the patients with blood stasis syndrome of unstable angina pectoris of coronary heart disease （CHD）,and discuss the relationship between ApoA-Ⅰ,α1-AT and inflammation.Methods The plasma samples were collected from the patients with the syndrome of qi deficiency and blood stasis or the syndrome of phlegm-stasis inter-obstruction （n=34） according strictly to the diagnostic standard of blood stasis syndrome of CHD unstable angina pectoris.The venous blood were collected from fast patients in the morning within 2 days after hospitalizing.After EDTA anticoagulation and centrifugation,the expressions of ApoA-Ⅰ and α1-AT were detected by using Western blotting method and enzyme-linked immunosorbent assay （ELISA）.Results The expression level of ApoA-Ⅰ was significantly lower,and the expression level of α1-AT was significantly higher in the group of syndrome of qi deficiency and blood stasis and group of syndrome of phlegm-stasis inter-obstruction than those in health control group （P0.05,P0.05）.Conclusion The expressions of ApoA-Ⅰ and α1-AT are different in the patients with blood stasis syndrome of CHD unstable angina pectoris,which indicates that inflammatory reaction may be one of mechanisms of blood stasis syndrome of CHD.

关 键 词：冠心病 血瘀证 炎症 载脂蛋白AⅠ- α_1-抗胰蛋白酶 

分 类 号：R259.414[医药卫生—中西医结合]