扫描近场光学显微镜对细胞超微结构的研究  

Study of Cell Ultrastructure by Scanning Near-field Optical Microscope

作　　者：李盛璞[1] 蔡继业[1] 

机构地区：[1]暨南大学生命科学学院化学系,广州510632

出　　处：《现代科学仪器》2010年第2期169-172,共4页Modern Scientific Instruments

基　　金：国家973重大项目(2010CB833603)

摘　　要：扫描近场光学显微镜(SNOM)突破了光学显微镜的衍射极限,在细胞研究中具有高灵敏性、无侵入性等优点,已经广泛的应用于生物学研究中。本文综述了SNOM在细胞膜、细胞器、细胞精细结构和单分子探测等领域的研究进展,介绍了扫描近场光学显微镜结合量子点的方法,并对其应用前景做了展望,对其面临困难做了概述。作为一种研究工具,SNOM在生物领域的应用还远远不足。Scanning near-field optical microscope（SNOM）has unique properties in the field of optical microscope. It breaks through optical diffraction limit,and has high sensitivity and non-invasive.SNOM has been widely used in biological research.This paper is the review about the SNOM research progress in the fields of the cell membrane, organelle,cell fine structure and single-molecule detection etc.The article focuses on the method that SNOM combines with quantum-dot and making outlook on application prospects.Now SNOM also faces a lot of difficulty,and its application is still not enough in the biological area.

关 键 词：扫描近场光学显微镜 单分子探测 分子生物学 量子点 

分 类 号：TH742[机械工程—光学工程]