PCR检测鼠疫菌pla基因的敏感性及特异性试验  

Sensitivity and Specificity of PCR Test for Detection of pla Gene of Yersinia pestis

作　　者：徐菲莉[1] 孙石[1] 赵飞[1] 

机构地区：[1]新疆地方病防治研究所

出　　处：《地方病通报》1998年第1期18-20,共3页Endemic Diseases Bulletin

基　　金：新疆维吾尔自治区科委资助

摘　　要：使用自己设计的一对引物，经ＰＣＲ检查鼠疫菌ｐｌａ基因。检查了５株鼠疫菌和假结核０５等４株近缘菌，结果显示：５株鼠疫菌均有阳性扩增带，而其它４株近缘菌则无，最少可检出１０ｆｇ的靶基因和１００ｃｆｕ，其敏感性略低于细菌学，但明显高于血清学。由于该法省时、省力，６小时即可完成，优于细菌学方法（２～７天）。By using a pair of selfdesigned primers for making PCR test, 5 strains of Yersinia pestis and 4 strains of such sibling bacteria as Yersinia pseudotuberculosis 05 were examined for pla gene of Yersinia pestis. Result shown all 5 strains of Yersinia pestis had amplification band of positive, 4 strains of such sibling bacteria were negative, and at least 10 fg of target gene and 100 cfu were detected. Its sensitivity was slightly lower than that of bacteriological method but significantly higher than that of serological method. This kind of testing method saves a lot of time and labour, being finished within 6 hours and proves to be superior to bacteriological method which usually lasts for 2～7 days. The use of this pair of primers for making PCR test is, therefore, practicable for rapid diagnosis of plague pathogens.

关 键 词：鼠疫菌 PCR 引物 敏感性 特异性 

分 类 号：R378.61[医药卫生—病原生物学]