组织块法分离山羊表皮干细胞研究  

Study on the Isolation of Goat Epidermal Stem Cells by Explant

作　　者：史明艳[1,2] 徐照学[3] 杨学义[1] 窦忠英[2] 

机构地区：[1]洛阳师范学院生命科学系,河南洛阳471022 [2]西北农林科技大学国家干细胞工程技术研究中心陕西分中心,陕西杨凌712100 [3]河南省农业科学院畜牧兽医研究所,河南郑州450002

出　　处：《河南农业科学》2010年第5期109-111,共3页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(30200137);国家“863”计划项目(2002AA216161);教育部重大科技专项项目(03160)

摘　　要：采用组织块法从山羊耳部皮肤基底层分离表皮干细胞,并通过形态学观察、免疫组化染色以及克隆形成率等方法检测山羊表皮干细胞在DMEM/F12和F12两种不同培养体系中的体外生长特性。结果表明,DMEM/F12是一种适合表皮干细胞体外增殖培养的培养基,在此培养体系中细胞可传代至11代。The epidermal stem cells were isolated from goat ear skin by epidermal explant and cultured in DMEM/F12 and F12 two different groups . The resultant cells were observed and identified by cell growth curve,immnohistochemical staining and colony-forming efficiency. The results showed that DMEM/F12 was a good cell culture medium for the epidermal stem cells and the epidermal stem cells can survive to the 11 subculture in vitro.

关 键 词：组织块 山羊 表皮干细胞 克隆形成率 

分 类 号：S827[农业科学—畜牧学]