OT型百合与O型百合远缘杂交试验  

Study on Distant Hybridization for OT Section Lily and O Section Lily Varieties

作　　者：张朝君[1] 郑思乡[1] 许超[2] 

机构地区：[1]贵州省园艺研究所贵州省园艺工程技术研究中心,贵州贵阳550006 [2]云南农业大学园林园艺学院,云南昆明650201

出　　处：《贵州农业科学》2010年第4期17-18,22,共3页Guizhou Agricultural Sciences

基　　金：贵州省农业科学院重点项目"OT×O型百合离体试管受精研究"[院ZX(2007)040];国家科技支撑计划项目"球根类花卉种质资源发掘和新品种研发"(2007BAD45B01);贵州省体改项目"贵州省花卉新品种培育及种苗繁育体系建设"[(2009)4006];贵州省基金项目"新铁炮百合与东方百合杂交技术的研究"[(2008)262];贵州省省长基金项目"LXOT及LXO新型百合远缘杂交技术的研究"[(2008)80];贵州省农业动植物育种专项"离体受精技术在百合远缘杂交育种中的应用"[(2009)031]

摘　　要：为获得OTO远缘杂交新材料，以OT型百合黄天霸（L．manissa）和东方百合品种西伯利亚（L．siberia）为试材，采用离体受精方法，不同授粉方式，结合胚挽救技术进行远缘杂交试验。结果表明：采用非常规授粉方法授粉后，获得了较高的子房膨大率；而常规授粉方法授粉后15～20d花瓣便枯萎，没有子房膨大的现象。黄天霸×西伯利亚采用子房切断的方法子房膨大率达到50％，西伯利亚×黄天霸采用柱头嫁接的方法，子房膨大率仅为14％。在正反交试验中，黄天霸×西伯利亚杂交效果比西伯利亚×黄天霸好，成功获得了OTO远缘杂交材料。In vitro fertilization, different pollination method, combination of embryo rescue were applied for the distant hybridization of OT section lily manissa and O section oriental lily L. siberia to obtain new OT × O distant hybridization breeding lily materials. Results showed that higher ovary enlargement ratio was obtained when unconventional pollination methods were applied. Conversely, petals were withered, after 15-20 days of conventional pollination, and no ovary enlargement observed. Higher ovary enlargement ratio （about 50%） was obtained by the application of ovary cutting method for L. mantissa× L. siberia, in contrast, only 14% of enlargement ratio was obtained by stigma grafting for L. siberia × L. mantissa. The effect of hybridization of L. mantissa ×L. siberia was better than L. siberia × L. mantissa in pros and cons hybridization experiments, new OT× O distant hybridization breeding lily materials were successfully obtained in the study.

关 键 词：OT型百合 东方百合 离体受精 远缘杂交 

分 类 号：S682.2[农业科学—观赏园艺] S603.2[农业科学—园艺学]