甘肃高山型细羊毛KRT35基因序列遗传多态性分析  

Analysis on Genetic Polymorphism of KRT35 Gene Sequence of Gansu Alpine Fine-wool Sheep

作　　者：王丽萍[1] 杨博辉[2] 岳耀敬[2] 牛春娥[2] 郭建[2] 孙晓萍[2] 郎侠[2] 刘建斌[2] 

机构地区：[1]甘肃农业大学动物科学技术学院,甘肃兰州730070 [2]中国农业科学院兰州畜牧与兽药研究所,甘肃兰州730050

出　　处：《贵州农业科学》2010年第4期142-144,148,共4页Guizhou Agricultural Sciences

基　　金：国家绒毛用羊产业技术体系建设专项"分子育种岗位"(nycytx-40-2);国家绒毛用羊产业技术体系建设专项子项目"绵羊新品种(系)培育"(BRF090102)

摘　　要：采用PCR-SSCP方法,检测甘肃高山型细毛羊优质毛品系111只KRT35CDS区部分序列的遗传多态性,并与羊毛弯曲度进行关联分析。结果表明:KRT35CDS区部分序列存在1个等位基因3种基因型。χ2检验表明,该群体在这一位点上处于Hardy-Weinberg平衡状态(P>0.05),多态信息含量(PIC)为0.4526,属于中度多态(0.5>PIC>0.25)。测序结果显示,与绵羊该基因序列(EU216426)相比,A等位基因在154处发生T→C的突变。关联分析表明:优质毛品系不同基因型之间羊毛弯曲度差异不显著。The genetic polymorphism of CDS partial sequence of KRT35 gene in 111 Gansu Alpine Fine-wool sheep were detected by PCR-SSCP method and the correlation between genotype and wool camber was analyzed. The results showed that there were one allele and three genotypes in CDS partial sequence of KRT35. The population was Hardy-Weinberg equilibrium at this locus （P〉0. 05） and the polymorphism information content was 0. 452 6. The sequencing result showed that the A allele had T-C mutation at the CDS partial sequence 154 bp. The correlation analysis indicated that the differences of wool camber among different genotypes were not significant.

关 键 词：甘肃高山型细毛羊 KRT35 SSCP 

分 类 号：S826.81[农业科学—畜牧学]