重庆5个地区PRRSV ORF7基因的克隆与序列分析  

Cloning and phylogenetic analysis of the ORF7gene of PRRSV from five regions in Chongqing

作　　者：宋苍浩[1] 楚常欢[1] 邸海波[1] 黄伟[1] 

机构地区：[1]重庆市西南大学荣昌校区,重庆荣昌402460

出　　处：《畜禽业》2010年第5期54-57,共4页Livestock and Poultry Industry

摘　　要：采用RT-PCR方法,对来自重庆合川、长寿、綦江、大足、开县等5个区县的5个猪场的PRRS病料进行了ORF7基因的扩增,并克隆、测序和序列比对。结果发现,这5株与美洲型代表毒株VR-2332的核苷酸序列同源性为98.5%,其推导的氨基酸序列同源性为92.6%;与已发表的ORF7序列CQ0703、JX0602、HB0602比对,核苷酸同源性为99.6%,其推导的氨基酸同源性为98.0%;与疫苗株RespPRRSMLV核苷酸同源性为98.6%,氨基酸同源性为94.3%,与欧洲型代表株LV株ORF7基因序列差异较大,核苷酸同源性仅有56.6%,而且推导的氨基酸数比LV株少5个,二者同源性仅为58.1%。结果表明5个区县流行的PRRSV与VR-2332和RespPRRSMLV亲缘关系很近,均为美洲型毒株。The ORF7 genes of PRRSV isolated from five pig farms in the county of Hechuan and Changshou and and Qijiang and Dazu and Kaixian in Chongqing in2009 were amplified by RT-PCR,and then clonging and sequencing,and blasting.It found that the nucleotide homology was 98.5% and 98.6%, respectively, and the Amino Acid homology was 92.6% and 94.3%, these five strains with VR-2332 and vaccines strain RespPRRS MLV,and with published sequence CQ0703,JX0602 and HB0602,the nucleotide and Amino Acid homology were 99.6% and 98.0% ,respectively,but there were much defferences between these five strain and European strain LV,the nucleotide and Amino Acid homology were 56.6%and58.1%, respectively,and its Amino acid was five numbers less than that of the LV strain .The results indicathed tha tthese strain isolated in Chongqing counties were closely related toVR-2332 and RespPRRS MIN, and also belonged to North American.

关 键 词：PRRSV ORF7 同源性 RT-PCR 

分 类 号：S852.65[农业科学—基础兽医学]