梨RAPD扩增产物两种凝胶电泳指纹特点及其序列分析  

Characteristics of Two Electrophoresis Systems and Sequences of RAPD Amplification Products of Pear

作　　者：王西成[1] 姜淑苓[2] 曹玉芬[2] 张彦平[1] 章镇[1] 房经贵[1] 

机构地区：[1]南京农业大学园艺学院,江苏南京210095 [2]中国农业科学院果树研究所,辽宁兴城125100

出　　处：《江西农业学报》2010年第4期1-5,8,共6页Acta Agriculturae Jiangxi

摘　　要：尽管RAPD技术已经应用于梨种质资源遗传多样性研究,但是尚没有对11个碱基引物的RAPD优化技术在梨品种鉴定方面的深入研究,也缺乏对梨RAPD的PCR产物特点进行分析的报道。鉴于此,该实验首次开展了选用11个碱基引物的RAPD鉴定梨品种上的研究,并对PCR扩增产物的琼脂糖凝胶以及聚丙烯酰胺凝胶(PAGE)电泳的指纹图谱加以比较分析。结果表明PAGE电泳检测出的谱带的总数量以及多态性谱带的数量均高于前者。两种电泳指纹标记资料进行梨品种聚类的结果存在一定差异。通过对随机挑取的21个片段进行克隆与测序分析,结果发现21个片段都是对应RAPD引物的扩增产物,其中3条为编码蛋白序列,18条为非编码蛋白序列。Although RAPD technique has been applied to the studies on the genetic diversity of pear germplasm resources,further studies on the identification of pear cultivars by use of optimized technology of 11 base pairs of RAPD primers had not yet been seen,and the analyses on the characteristics of RAPD amplification products of pear were very few.So the selected 11 base primers were used to analyze the genetic relationship of various pear cultivars with RAPD technique in this paper for the first time.The results were analyzed by agarose and polyacrylamide gel electrophoresis.The comparative results showed that polyacrylamide gel could discriminate more total bands and polymorphic bands than agarose gel.There were some differences between the two polygenetic trees which derived from the analysis results.Twenty-one random fragments were cloned and sequenced.The results showed that all the fragments were the PCR amplification products from the corresponding primer.Three sequences among them were protein-coding genes and the others were non-coding sequences.

关 键 词：梨 RAPD 凝胶电泳 多态性 

分 类 号：S661.2[农业科学—果树学]