猪GRIFIN基因克隆与组织分布  

Molecular Cloning and Tissue Distribution of Gene GRIFIN in Piglet

作　　者：孙娅莉[1] 李宏基[1] 韩立强[1] 王伟杰[1] 常磊[1] 郭豫杰[1] 杨国宇[2] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002 [2]农业部动物生长发育调控重点开放实验室,河南郑州450002

出　　处：《江西农业学报》2010年第4期15-17,共3页Acta Agriculturae Jiangxi

基　　金：河南省重大科技攻关项目(0522010800)

摘　　要：从仔猪的眼球中克隆GRIFIN基因,研究GRIFIN在仔猪不同组织中的分布情况。结果成功克隆出猪GRIFIN基因,全长466 bp,包含一个完整的开放阅读框架432 bp,编码144个氨基酸。组织分布结果显示GRIFIN在断乳仔猪多种组织均有分布。The coding sequence of GRIFIN was cloned from the tissue of piglet.The gene expression of GRIFIN in various piglet tissues was amplified by RT-PCR.The result showed that the full length of GRIFIN was 466 bp,including an open reading flame of 432 bp,encoding 144 amino acids.Semi-quantitative RT-PCR analysis showed that GRIFIN mRNA could ubiquitously express in various piglet tissues tested.

关 键 词：仔猪 GRIFIN 基因克隆 组织分布 

分 类 号：S828[农业科学—畜牧学]