1,1-二氯乙烯降解菌的分离鉴定及降解特性  

Isolation and identification of a 1,1-dichloroethylene-degrading bacterial strain and its degradation characteristics

作　　者：周雪[1] 唐顺[1] 李冬梅[1] 杨琦[1] 尚海涛[1] 郝春博[1,2] 

机构地区：[1]中国地质大学(北京)水资源与环境学院,北京100083 [2]中国地质大学(北京)地质微生物实验室,北京100083

出　　处：《环境工程学报》2010年第5期983-987,共5页Chinese Journal of Environmental Engineering

基　　金：国家自然科学基金资助项目(40102027;50578151);北京市自然科学基金资助项目(8052017;8092025);北京市教委产学研项目(51900265005);国家水体污染控制与治理重大专项资助(2009ZX07419);国家科技重大专项(2009ZX07207-008;2009ZX07419-002;2009ZX07207-001)

摘　　要：从好氧活性污泥中分离得到一株能以1,1-二氯乙烯（1,1-DCE）作为惟一碳源和能源生长的革兰氏阴性菌株D-B,经鉴定属于产碱杆菌属（Alcaligenessp.）。当维持菌株D-B浓度一定时,1,1-DCE的去除率随着1,1-DCE浓度的增大先增加后降低,且降解过程主要发生在加入1,1-DCE后的3~5 h内。当1,1-DCE的初始浓度为300μg/L时去除率达到最大值85.32%。菌株D-B对1,1-DCE的降解符合Monod方程,饱和常数Ks=21.96 mg/L,1,1-DCE的最大比基质降解速率Vmax=50.76 mg/（L.h）。A Gram-negative bacterial strain D-B,which was able to grow on 1,1-dichloroethylene（1,1-DCE） as sole carbon and energy source,was isolated from aerobic activated sludge and identified as Alcaligenes sp.When maintaining the same concentration of strain D-B,the biological removal rate of 1,1-DCE increased at first and then decreased with the increasing 1,1-DCE and the degradation was mainly taken place within 3 to 5 hours after adding 1,1-DCE.The removal rate of 1,1-DCE attained the maximum value of 85.32% when the initial concentration of 1,1-DCE was 300 μg/L.The removal of 1,1-DCE degraded by strain D-B was in accordance with the Monod equation： the saturation constant was Ks=21.96 mg/L and the maximum specific substrate degradation rate of 1,1-DCE was Vmax=50.76 mg/（L·h）.

关 键 词：1 1-二氯乙烯生物降解分离鉴定动力学Monod方程 

分 类 号：O623.221[理学—有机化学]