有机添加物对矮败小麦F_1可育株花药培养及花培苗越夏的影响  

The effect of the organic additives on the characteristics of F_1 fertile anther materials of dwarf-male-sterile wheat and on summering of cultured wheat anther plantlets

作　　者：史勇[1] 白延红[2] 陈耀锋[1] 李春莲[1] 崔志刚[1] 安娜[1] 李晓飞[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100 [2]杨凌职业技术学院生物工程系,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2010年第5期73-79,共7页Journal of Northwest A&F University(Natural Science Edition)

基　　金：陕西省13115科技重大专项(2008ZDKG-07)

摘　　要：【目的】探讨有机添加物对矮败小麦F1可育株花药培养特性的影响,研究其花粉植株越夏方法。【方法】应用单因子试验,分析水解酪蛋白、谷氨酰胺和生物素3种有机添加物对矮败小麦F1可育株可育株花药培养特性的影响,比较不同基因型矮败小麦F1可育株花药愈伤组织诱导率、绿苗分化率,研究多效唑对矮败小麦F1可育株花粉植株越夏的影响。【结果】在一定质量浓度范围内,水解酪蛋白、谷氨酰胺和生物素对矮败小麦F1可育株花药愈伤组织的诱导都有一定正向促进作用,在其最适质量浓度(水解酪蛋白500 mg/L、谷氨酰胺5.0 mg/L和生物素2.0 mg/L)时,矮败小麦F1可育株花药愈伤组织的诱导率分别较对照提高了47.07%,22.48%和55.27%;基因型对矮败小麦F1可育株花药愈伤组织诱导和绿苗再分化有重要影响,矮败小麦与小偃22、101、西农213 3个小麦品种(系)杂交F1可育株花药愈伤组织诱导率分别为5.49%,4.73%和5.85%,三者之间差异均达极显著水平。矮败×小偃22愈伤组织的绿苗分化率明显高于矮败小麦与101、西农213杂交F1代2个供试材料,达到了13.70%。在添加多效唑和连续继代培养条件下,成功地进行了矮败小麦F1花粉植株越夏,证明在越夏培养过程中,4.0 mg/L多效唑表现出的延缓生长作用较3.0 mg/L更好,并未见有毒害作用。【结论】添加500 mg/L水解酪蛋白、5.0 mg/L谷氨酰胺和2.0 mg/L生物素,能使矮败小麦F1可育株花药愈伤组织的诱导率较对照有明显提高;添加多效唑和连续继代培养可使矮败小麦F1花粉植株成功越夏,获得生长健壮、移栽成活率高的花培苗。【Objective】 This experiment discussed the effect of the organic additives on the F1 fertile anther materials of dwarf-male-sterile wheat and studied the summering of cultured wheat anther plantlets.【Method】 Single-factor test was used to analyze the effect of hydrolisis casein,L-glutamine,and biotin on the culture characteristics of dwarf-male-sterile F1 fertile plant anthers,the three different genotype materials＇callus induction rates and green plant regeneration rates were respectively compared,and the effect of PP333 on dwarf-male-sterile F1 fertile plants over-summering was analyzed.【Result】 In certain concentrations,hydrolisis casein,L-glutamine,and biotin on culturing the dwarf-male-sterile wheat F1 anthers all had certain positive effects on promoting F1 anther callus.In their optimum concentrations（respectively：500,5.0,2.0 mg/L）,compared with the control,the induction rate of the anther callus was increased respectively by 47.07%,22.48% and 55.27%;Different materials＇ genotypes had different effects on the callus induction and green plantlet differentiation of the dwarf-male-sterile wheat F1 anthers,dwarf-male-sterile hybrid with Xiaoyan 22,101,Xinong 213 respectively,and the anther callus rate of their F1 generations was 5.49%,4.73% and 5.85% respectively,the difference among which was extremely significant.The green plantlet differentiation of callus of dwarf-male-sterile×Xiaoyan 22 reached 13.70%,which was significantly higher than that of dwarf-male-sterile ×101 and dwarf-male-sterile×Xinong 213.The cultured green wheat anther plantlets passed the summer safely on the condition of continuous subculture and certain concentration of PP333 was put into the media.The 4.0 mg/L concentration of PP333 showed better delay growing role than 3.0 mg/L,and 4.0 mg/L concentration of PP333 did not harm the plantlets.【Conclusion】 After adding hydrolyzed casein,L-glutamine and biotin whose mass concentrations were 500,5.0,2.0 mg/L respectively,the anther callus induction rates of dwarf m

关 键 词：矮败小麦 花药培养 有机添加物 多效唑 

分 类 号：S512.19[农业科学—作物学] Q813.12[生物学—生物工程]