舒冠滴丸对高脂血症大鼠缺血再灌注损伤心肌MPO活性及中性粒细胞浸润的影响研究  

The influence of shuguan dripping pill on MPO activity and the infiltration of neutrophils with myocardial ischemia reperfusion injury in hyperlipidemic rats

作　　者：王建湘[1] 程丑夫[1] 邓满霞[1] 

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《中医临床研究》2010年第9期34-36,共3页Clinical Journal Of Chinese Medicine

摘　　要：目的：观察舒冠滴丸对高脂血症大鼠缺血再灌注损伤心肌MPO活性及中性粒细胞浸润的影响及其保护作用机制探讨。方法：建立大鼠高脂血症模型，分为对照组、假手术组、模型组及舒冠滴丸高、中和低剂量组以及辛伐他汀组。再灌注后，测定心肌梗死面积、心肌组织MPO含量及中性粒细胞浸润情况，观察心肌病理形态学变化。结果：与模型组比较，舒冠滴丸高剂量组和中剂量组心肌梗死面积与左心室面积比值及MPO活性均降低（P〈0．05）；舒冠滴丸高剂量组和辛伐他汀组缺血区心肌纤维断裂及坏死减少，空泡变性减轻，灶性出血及中性粒细胞浸润减轻。结论：舒冠滴丸对高脂血症大鼠心肌缺血再灌注损伤有一定的保护作用，其机制可能与降低大鼠心肌组织MPO活性、减轻PMN浸润，抑制炎症反应有关。Objective： To investigate the effects of Shuguan Dripping Pill on the activity of myeloperoxide（MPO） and the infiltration of neutrophils with myocardial ischemia reperfusion injury in hyperlipidemic rats and its mechanisms. Methods： After hyperlipidemic rat models were set up by oral administration of high lipid emulsion, the rats were divided into seven groups： control group,sham group, model group, large,middle, low dosage of Shuguan Dripping Pill groups and Simvastatin group. After reperfusion, MPO activities of ischemic myocardium were measured; HE staining was used to observe infiltrating neutophils; Infarct area of left ventricles was measured by Evens blue-TTC staining; and myocardial pathological changes were also observed. Results： Compared with model group, the myocardial infarct area/ventricle area ratios and MPO activities in both large and middle dosge of Shuguan Dripping Pill groups decreased （P〈0.05）; In both large dosage of Shuguan Dripping Pill and Simvastatin groups myocardial fiber rupture and necrosis in ischemic area lessened, focal hemorrhage and the infiltration of neutrophils decreased. Conclusion Shuguan Dripping Pill has protective effects on myocardial ischemia reperfusion injury in hyperlipidemic rats and the mechanisms involved may be related to its decrease of MPO activity and neutrophil infiltration in myocardium, and inhibition of inflammatory action.

关 键 词：舒冠滴丸 心肌缺血再灌注损伤 高脂血症 髓过氧化物酶 中性粒细胞 

分 类 号：R589.2[医药卫生—内分泌]