疫霉根腐病菌毒素对大豆不同组织中多酚氧化酶的影响  

Changes of Polyphenol Oxidase in Soybean Treated by Pathotoxin Produced by Phytophthora sojae

作　　者：徐鹏飞[1] 王萍[2] 吴俊江[3] 张淑珍[1] 范素杰[1] 李宁辉[1] 王欣[1] 陈晨[1] 李文滨[1] 

机构地区：[1]东北农业大学大豆研究所教育部大豆生物学重点实验室,黑龙江哈尔滨150030 [2]淮海工学院海洋学院,江苏连云港222005 [3]黑龙江省农业科学院大豆研究所,黑龙江哈尔滨150086

出　　处：《作物杂志》2010年第2期28-31,共4页Crops

基　　金：国家自然科学基金(30400285;30671317;30810103063;30972889);黑龙江省自然科学基金(C200814);中国博士后基金(20060400835);黑龙江省新世纪优秀人才培养计划(NCET-06-007);黑龙江省高校骨干教师项目(1151G007);黑龙江省农科院博士后基金(LRB06-010);博士后落户黑龙江科研启动基金(LBH-Q05032);黑龙江省青年基金(QC06C012);农业部寒地重点开放实验室开放课题

摘　　要：大豆疫霉根腐病是危害大豆生产的世界范围的毁灭性病害,毒素在其致病过程中起着重要的作用。本文对大豆疫霉根腐病菌毒素胁迫下抗感不同大豆品种根、茎、叶中多酚氧化酶(PPO)活性的变化进行了初步研究,结果表明:适宜浓度的毒素(稀释100倍,浓度为0.0897mg/mL)处理后,抗病品种根、茎和叶中PPO活性在病程的大部分阶段与对照相比都升高,并且根中的PPO比茎叶中的PPO反应更敏感;而感病品种在整个病程中虽然在某些阶段较对照有一定的提高,但幅度不大,在病程其他阶段PPO下降幅度远大于升高幅度。而浓度相对较高的毒素(稀释50倍,浓度为0.1794mg/mL)处理后抗感品种根、茎和叶中PPO活性的变化较稀释100倍浓度毒素幅度小。Phytophthora root and stem rot caused by Phytophthora sojae is a destructive soybean disease worldwide,and the pathotoxin produced from P.sojae is an important pathogenic factor.In this study,changes of Polyphenol Oxidase（PPO）of nine soybean varieties with different resistance treated by the pathotoxin was investigated.The results showed that the PPO activity in roots,stems,and leaves of resistant soybean varieties treated by the pathotoxin of optimal concentration（Diluted to 100 times,and the concentration is 0.0897 mg/mL）was higher than that of the control at most of the pathogenic stages.The reaction of PPO in roots was more sensitive than that in stems and leaves.The PPO activity in susceptible soybean varieties increased compared with that in the control at some of the pathogenic stages,but decreased at most of the pathogenic stages.Treated with pathotoxin with high concentration（Diluted to 50 times,and the concentration is 0.1794 mg/mL）,the change range of PPO activity in roots,stems and leaves in the nine soybean varieties was relatively low.

关 键 词：大豆 大豆疫霉根腐病 毒素 多酚氧化酶 

分 类 号：S565.1[农业科学—作物学]