Cloning and Expression Level Analysis of Two BnaANT Candidate Genes in Brassica napus  

作　　者：CHEN Bo WANG Tai-xia WANG Han-zhong LI Yun-chang YAN Xiao-hong WANG Li-jun WEIWen-hui 

机构地区：[1]Institute of Oil Crops, Chinese Academy of Agricultural Sciences/Key Laboratory for Biological Sciences of Oil Crops, Ministry of Agriculture, Wuhan 430062, P.R.China [2]College of Life Sciences, Henan Normal University, Xinxiang 453007, P.R.China

出　　处：《Agricultural Sciences in China》2010年第4期488-496,共9页中国农业科学（英文版）

基　　金：supported by the National Natural Sci-ence Foundation of China (30671312);the Natural Sci-ence Foundation of Hubei Province, China(2008CDA083);the Chenguang Program of Wuhan City, China (200750731300)

摘　　要：AINTEGUMENTA （ANT） gene has been proved to have a novel function on controlling the organ size and the seed weight in Arabidopsis thaliana, the research on its orthologous gene in Brassica napus will be of potential interest to elucidate the molecular mechanism of rapeseed development and increase the rapeseed output. A SMART cDNA library of the flower bud from B. napus cultivar Zhongshuang 9 was constructed, and the full-length cDNAs of two homologous BnaANT candidate genes were cloned by PCR in B. napus, namely BnaX.ANT.a （GenBank accession no. DQ211969） and BnaX.ANT.b （accession no. DQ211970）. They shared 87.1 and 83.4% amino acid identity with ANT of A. thaliana, respectively. There is 87.4% amino acid identity between BnaX.ANT.a and BnaX.ANT.b. By quantitative real-time PCR, the obviously differential expression levels of the two BnaANT candidate genes were detected in the 28 DAF seeds of the big and small grains B. napus lines, the expression abundance of BnaANT in the 28 DAF seed of 9311 was over three times compared to that of 9260, which indicates that these two BnaANT genes are also likely related to the floral organ size and the seed weight in B. napus.AINTEGUMENTA （ANT） gene has been proved to have a novel function on controlling the organ size and the seed weight in Arabidopsis thaliana, the research on its orthologous gene in Brassica napus will be of potential interest to elucidate the molecular mechanism of rapeseed development and increase the rapeseed output. A SMART cDNA library of the flower bud from B. napus cultivar Zhongshuang 9 was constructed, and the full-length cDNAs of two homologous BnaANT candidate genes were cloned by PCR in B. napus, namely BnaX.ANT.a （GenBank accession no. DQ211969） and BnaX.ANT.b （accession no. DQ211970）. They shared 87.1 and 83.4% amino acid identity with ANT of A. thaliana, respectively. There is 87.4% amino acid identity between BnaX.ANT.a and BnaX.ANT.b. By quantitative real-time PCR, the obviously differential expression levels of the two BnaANT candidate genes were detected in the 28 DAF seeds of the big and small grains B. napus lines, the expression abundance of BnaANT in the 28 DAF seed of 9311 was over three times compared to that of 9260, which indicates that these two BnaANT genes are also likely related to the floral organ size and the seed weight in B. napus.

关 键 词：AINTEGUMENTA gene Brassica napus BnaANT gene yield-related gene quantitative real-time PCR 

分 类 号：Q78[生物学—分子生物学] S565.4[农业科学—作物学]