Effects of Pretreatment by the Flavanol Ampelopsin on Porcine Kidney Epithelial Cell Injury Induced by Hydrogen Peroxide  

作　　者：TAN Gao-yi ZHANG Min-hong FENG Jing-hai HAN Ai-yun ZHENG Shan-shan XIE Peng 

机构地区：[1]State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China

出　　处：《Agricultural Sciences in China》2010年第4期598-604,共7页中国农业科学（英文版）

基　　金：supported by the National Basic Re-search Program of China (2004CB117507);supportprovided by the Key Technologies R&D Program of China during the 11th Five-Year Plan period(2006BAD14B02-8)

摘　　要：This study investigated the protective effects of ampelopsin on H2O2-induced oxidative injury in porcine kidney epithelial cell line, PK-15; cells were pretreated with medium containing 0, 15, 30, and 60 ug mL-1 ampelopsin, respectively, for 1 h prior to the addition of 100 umol L-1 H2O2. After 2 h, the cell viability, intracellular superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） activity, cellular malondialdehyde （MDA） content, and DNA strand breakage were determined. Results showed that ampelopsin pretreatment did not modify the SOD and GSH-Px inactivation but significantly decreased MDA production; except for the medium dose （30 ug mL-1）, all the tested concentrations of ampelopsin did not significantly inhibit cell viability reduction and gave no influence on DNA damage. The results suggested that all the tested doses of ampelopsin pretreatment before H2O2 addition can protect PK-15 cell from H2O2-induced lipid peroxidation, which might not be mediated by the SOD and GSH-Px responsees. The appropriate dose of ampelopsin pretreatment prevents PK-15 cell from DNA damage and cell viability reduction.This study investigated the protective effects of ampelopsin on H2O2-induced oxidative injury in porcine kidney epithelial cell line, PK-15; cells were pretreated with medium containing 0, 15, 30, and 60 ug mL-1 ampelopsin, respectively, for 1 h prior to the addition of 100 umol L-1 H2O2. After 2 h, the cell viability, intracellular superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） activity, cellular malondialdehyde （MDA） content, and DNA strand breakage were determined. Results showed that ampelopsin pretreatment did not modify the SOD and GSH-Px inactivation but significantly decreased MDA production; except for the medium dose （30 ug mL-1）, all the tested concentrations of ampelopsin did not significantly inhibit cell viability reduction and gave no influence on DNA damage. The results suggested that all the tested doses of ampelopsin pretreatment before H2O2 addition can protect PK-15 cell from H2O2-induced lipid peroxidation, which might not be mediated by the SOD and GSH-Px responsees. The appropriate dose of ampelopsin pretreatment prevents PK-15 cell from DNA damage and cell viability reduction.

关 键 词：AMPELOPSIN PK-15 cell ANTIOXIDATION hydrogen peroxide 

分 类 号：Q813.11[生物学—生物工程] TQ123.6[化学工程—无机化工]