不同产地野菊花中绿原酸、咖啡酸和蒙花苷含量  

Determination of chlorogenic acid,caffeic acid and linarin in Flos Chrysanthemi Indici from different places by RP-HPLC

作　　者：郭巧生[1] 房海灵[1] 申海进[1] 

机构地区：[1]南京农业大学中药材研究所,江苏南京210095

出　　处：《中国中药杂志》2010年第9期1160-1163,共4页China Journal of Chinese Materia Medica

基　　金：国家科技基础条件平台项目(2005DKA21000)

摘　　要：目的:对22份不同产地野菊花中绿原酸、咖啡酸和蒙花苷的含量进行测定,为科学评价及有效控制其质量提供参考。方法:采用Shim-pack C18色谱柱(4.6mm×250mm,5μm);绿原酸和咖啡酸流动相为乙腈-0.5%磷酸水溶液(19∶81);检测波长326nm。蒙花苷流动相甲醇-水-冰醋酸(26∶23∶1);检测波长334nm,柱温25℃;流速1mL·min-1。结果:绿原酸、咖啡酸和蒙花苷的浓度与峰面积,分别在2.5～50μg(r=0.998),2.5～25μg(r=0.998),4.97～41.47μg(r=0.999)呈良好的线性关系;平均加样回收率分别为100.8%,96.2%,103.7%,RSD分别为2.1%,2.3%,1.8%。结论:不同产地野菊花中绿原酸、咖啡酸和蒙花苷含量存在显著差异,重庆丰都产野菊花中绿原酸含量最高为0.232%,咖啡酸含量整体偏低,江苏和安徽产野菊花中蒙花苷含量基本达到药典规定水平。Objective： To evaluate the quality of Flos Chrysanthemi Indici which produced in twenty-two different producing places. Method： Chlorogenie acid and caffeie acid were analyzed on a Shim-pack C18 colunm （4. 6 mm × 250 mm, 5 μm） eluted with the mobile phase consisted of acetonitrile-0. 5% phosphoric acid（ 19：81 ）. The detection wavelength was set at 326 nm. Linarin were eluted with the mobile phase consisted of methanol-water-acetic acid（26： 23：1 ）. The detection wavelength was set at 334 nm. The column temperature was 25 ℃. The flow rate was 1.0 mL · min^-1. Result： The linear response ranged within 2. 5-50 μg for chlorogenic acid（ r = 0. 998 ） , 2. 5-25μg for caffeic acid （ r = 0. 998 ） and 4. 97-41.47 μg for linarin （ r = 0. 999 ） , respectively. Recoveries were 100. 8% with RSD 2. 1% for chlorogenic acid, 96. 2% with RSD 2. 3% for caffeic acid and 103.7% with RSD 1.8 % for linarin. Conclusion： There was a significant difference in the content of chlorogenic acid, caffeie acid, tinarin among the samples. The content of ehlorogenic in the sample from Fengdou Chongqing city was the highest in those from other places. The content of eaffeie acid in the all samples is very low. The content of linarin in the samples from Jiangsu province and Anhui province almost reached the national standard in pharmacopoeia.

关 键 词：野菊花 绿原酸 咖啡酸 蒙花苷 高效液相色谱法 

分 类 号：R284[医药卫生—中药学]