外源硝酸钙对盐胁迫下黄瓜幼苗叶片抗氧化系统及膜质子泵活性的影响  

Effects of exogenous Ca on leaf antioxidant system and membrane proton pump activity of cucumber seedlings under salt stress

作　　者：程玉静[1] 郭世荣[1] 刘书仁[1] 田婧[1] 刘超杰[1] 

机构地区：[1]南京农业大学园艺学院,农业部南方蔬菜遗传改良重点开放实验室,南京210095

出　　处：《生态学杂志》2010年第5期892-898,共7页Chinese Journal of Ecology

基　　金：国家重点基础研究发展计划项目(2009CB119000);现代农业产业技术体系建设专项基金;江苏省农业三项工程资助项目(SX(2008)026)

摘　　要：采用营养液水培法,以较耐盐黄瓜品种"新泰密刺"为试验材料,研究了叶面喷施硝酸钙对盐胁迫(NaCl65mmol·L-1)下黄瓜幼苗活性氧、谷胱甘肽-抗坏血酸循环(GluAsA)中抗氧化酶和抗氧化物质及膜质子泵活性的影响。结果表明:叶面喷施硝酸钙能够显著降低盐胁迫下黄瓜幼苗叶片超氧阴离子(O.2-)产生速率和丙二醛(MDA)含量;显著提高盐胁迫下黄瓜幼苗叶片抗坏血酸过氧化物酶(APX)和脱氢抗坏血酸还原酶(DHAR)等酶活性、抗氧化物质抗坏血酸(AsA)含量、AsA/DHA和GSH/GSSG比值及质膜和液泡膜H+-ATPase和H+-PPase活性。表明外源硝酸钙通过提高Glu-AsA抗氧化系统和膜质子泵活性,降低活性氧对叶片的伤害,增强了植株抗氧化能力和对离子的区域化,进而提高植株盐胁迫耐性。Taking salt-tolerant cucumber （Cucumis sativus L.） variety ‘Xintai Mici’ as test object, a water culture experiment was conducted to study the effects of foliar spraying Ca（NO3）2 on leaf reactive oxygen species （ROS）, antioxidant enzyme activities, antioxidants, and membrane proton pump activity of cucumber seedlings under the stress of 65 mmol NaCl·L^-1. Under this stress, foliar spraying Ca（NO3）2 decreased the O2^- production rate and MDA content, and increased the APX and DHAR activities, AsA content, AsA/DHA and GSH/GSSG ratios, and membrane H^＋-ATPase and H^＋-PPase activities significantly, suggesting that exogenous Ca could mitigate the oxidative damage induced by salt stress, and enhance the salt-tolerance of plants through up-regulating the plant antioxidant ability and ion compartmentation.

关 键 词：盐胁迫 黄瓜 硝酸钙 抗氧化系统 质子泵活性 

分 类 号：S642.2[农业科学—蔬菜学] Q945[农业科学—园艺学]