支气管灌注Fe_2O_3纳米粒对大鼠肺组织影响  

Effect of intratracheal instillation of Fe_2O_3 nanoparticles on lung tissue in rats

作　　者：杨扬[1] 唐萌[1] 张婷[1] 陆敏玉[1] 王宜庆[1] 孔璐[1] 薛玉英[1] 张宇[2] 顾宁[2] 

机构地区：[1]东南大学公共卫生学院劳动与环境卫生教研室(环境医学工程教育部重点实验室、江苏省生物材料与器件重点实验室),南京210009 [2]东南大学生物医学与医学工程系

出　　处：《中国公共卫生》2010年第5期572-573,共2页Chinese Journal of Public Health

基　　金：国家重大基础研究“973”计划(2006CB705602);国家自然科学基金(3067182,3092504)

摘　　要：目的观察支气管灌注Fe2O3颗粒对大鼠肺组织的氧化损伤及致纤维化作用。方法雄性大鼠经支气管灌注单次染毒(7 d)及多次染毒(30 d)不同剂量的20和280 nm Fe2O3粒子悬浮液,测定其肺组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽-过氧化物酶(GSH-Px)、羟脯氨酸(HYP)含量。结果20 nm Fe2O340 mg/kg剂量组大鼠肺组织中SOD(32.5±3.8)U/(mg.prot)、MDA(2.29±0.18)nmol/(mg.prot)及GSH-Px(80.4±9.61)U(mg.prot)含量明显高于对照组(P<0.05);除30 d的GSH-Px外,20 nm高剂组的SOD、MAD及GSH-Px含量均高于280 nm高剂量组(P<0.05),表明纳米级Fe2O3颗粒比微米级损伤更为严重。结论20 nm Fe2O3可致大鼠肺脏氧化损伤且较280 nm Fe2O3更为严重,但无明显致纤维化作用。Objective To study oxidative damage and fibrosis of lung tissue caused by intratiracheally instilling Fe2O3 nanoparticles in rats.Methods Male rats were given different doses of 20 nm Fe2O3 and 280 nm Fe2O3 suspension by intratracheal instillation.Some rats were given single-exposure then sacrificed at the end of 7 days.The other rats were repeatedly exposed and sacrificed at the end of 30 days.Superoxide dismutase（SOD）,malondialdehyde（MDA）,glutathione-peroxidase（GSH-Px）,and hydroxyproline（HYP） in tissue of the rats were detected.Results The levels of SOD（32.5±3.8 U/mg prot）,MDA（2.29±0.18 nmol/mg prot） and GSH-Px（80.4±9.61 U/mg prot） in the lung tissue of the rats in high-dose group（20 nm Fe2O3） were significantly different form those of control group（P0.05）.The levels of SOD,MDA and GSH-Px in lung tissue of the rats in high-dose of 20 nm Fe2O3 group were significantly higher than those of high-dose of 280 nm Fe2O3 group,except GSH-Px in the rats of 30 days exposure,indicating severe damage caused by Fe2O3 nanoparticles.The levels of MDA and GSH-Px in the rats of high-dose group showed significant difference between the groups of 7 days＇e and and 30 days＇ exposure.Conclusion The oxidative damage caused by 20 nm Fe2O3 in rat lungs is more serious than that caused by the 290 nm Fe2O3.No obvious fibrosis is found.

关 键 词：纳米粒 FE2O3 支气管灌注 氧化损伤 肺纤维化 

分 类 号：R332.2[医药卫生—人体生理学]