20种中药水溶性糖HPLC指纹图谱与寒热药性关系研究  

Relationship between high performance liquid chromatographic fingerprints of water-soluble sugar of 20 kinds of traditional Chinese herbs and both cold and heat properties

作　　者：李静文[1] 李峰[1] 周正礼[1] 

机构地区：[1]山东中医药大学药学院,山东济南250355

出　　处：《山东中医药大学学报》2010年第3期195-199,共5页Journal of Shandong University of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划("973"计划)项目(编号:2007CB512601);山东省科技发展计划课题(编号:2006GG2202017;2006GG3202003)

摘　　要：目的:探讨中药寒热药性与水溶性糖的相关性,进一步阐明中药四性与其物质基础的关系。方法:选取10种寒性中药和10种热性中药,提取水溶性糖,加入1-苯基-3-甲基-5-吡唑啉酮进行衍生化反应,测定水溶性糖的HPLC指纹图谱,利用Fisher方法建立判别函数,分析实验结果。结果:建立寒热药性的Fisher判别函数,实现了对20种中药寒热药性的准确判别,判别正确率为100%。结论:中药寒热药性与水溶性糖存在明显的相关性,Fisher可以作为判别中药寒热药性与水溶性糖关系的统计学工具,水溶性糖是中药寒热药性的物质基础之一。Objective：To discuss the correlation between both cold and heat properties and water-soluble sugar,and reveal the relation of four properties and their material basis. Methods：Extracting water-soluble sugar respectively from 10 kinds of cold herbs and heat herbs,1-phenyl-3-methyl-5-pyrazolone were added to the liquid for derivative reaction. The HPLC fingerprints of water-soluble sugar were analyzed by Fisher method through establishing discriminant function. Results ：Fisher dicriminant function was established for both cold and heat properties,which confirmed that there were many differences between the cold and heat properties in the HPLC fingerprints. The group back substitution was 100%. Conclusion：There was a significant relationship between both cold and heat properties and water-soluble sugar. Fisher analysis could distinguish the cold and heat properties of TCM. In addition,the water-soluble sugar was one of the material basis of four properties of TCM.

关 键 词：寒热药性 水溶性糖 HPLC 指纹图谱 Fisher分析 

分 类 号：R285.5[医药卫生—中药学]