电针对MCAO模型大鼠海马CA1区P-gp表达的影响  

Effects of electro-acupuncture on P-gp expression in hippocampal CA1 region in rats with MCAO

作　　者：刘红[1] 宋修涛[1] 卢岩[2] 王世军[2] 

机构地区：[1]山东中医药大学 [2]山东中医药大学针灸推拿学院,山东济南250355

出　　处：《山东中医药大学学报》2010年第3期260-262,共3页Journal of Shandong University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(编号:30672717);山东省科技发展计划课题(编号:2006-202017;2006-02003)

摘　　要：目的:研究电针内关对局灶性脑缺血模型大鼠海马CA1区微血管内皮细胞P-gp表达的影响,探讨针刺治疗脑缺血的机制。方法:采用大脑中动脉栓塞法(MCAO)复制局灶性脑缺血模型,电针内关、地机,应用免疫组化法观察各组大鼠海马CA1区P-gp的表达。结果:MCAO模型大鼠海马CA1区微血管内皮细胞MDR1表达下降,30d时表达与15d相比明显增多,有显著性差异(P<0.05),表现为时间依赖性升高。针刺内关穴后P-gp的表达显著增多,并且针刺天数不同疗效也不同。结论:针刺对MCAO大鼠海马CA1区P-gp的表达有良性调节作用。Objective：To study effects of electro-acupuncture on the expression of MDRl-encoded P-gp（P-gp） of microvaseular endothelial cells in hippoeampal CA1 region in focal cerebral ischemia rats and to investigate the mechanism of acupuncture on cerebral ischemie diseases. Methods：Middle cerebral artery occlusion（MCAO） models were made by blocked middle cerebral artery,then Neiguan and Diji were stimulated by electric acupuncture. The expression of P-gp in cerebral mierovaseular endothelial cells in hippoeampal CA1 region was observed with the method of immunohistoehemistry. Results：After model,the expression of MDR1 of mierovascular endothelial cells were down-regulated. Those were increased at 30th day treated by electric-acupuncture. There was obvious deference with the expression at 15th day（P〈0.05）. The expression of P-gp showed time-dependent tendency. The expression of P-gp increased significantly after acupuncture at Neiguan acupiont,and this effect was varying in different days with acupuncture. Conclusion：There were positive regulatory effect of aeupuncture on the expression of P-gp in hippocampal CA I region of MACO rat.

关 键 词：电针 内关 局灶性脑缺血 海马 P—gp 大鼠 

分 类 号：R245.9[医药卫生—针灸推拿学]