Anaerobic benzene biodegradation by a pure bacterial culture of Bacillus cereus under nitrate reducing conditions  

作　　者：Junfeng Dou Aizhong Ding Xiang Liu Yongchao Du Dong Deng Jinsheng Wang 

机构地区：[1]College of Water Sciences, Beijing Normal University, Engineering Research Center of Groundwater Pollution Control and Remediation, Ministry of Education, Beifing 100875, China. [2]Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China

出　　处：《Journal of Environmental Sciences》2010年第5期709-715,共7页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (No. 40873076,40773055);the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry (No.2008890)

摘　　要：A pure culture using benzene as sole carbon and energy sources was isolated by screening procedure from gasoline contaminated soil.The analysis of the 16S rDNA gene sequence,morphological and physiological characteristics showed that the isolated strain was a member of genus Bacillus cereus.The biodegradation performance of benzene by B.cereus was evaluated,and the results showed that benzene could be efficiently biodegraded when the initial benzene concentration was below 150 mg/L.The metabolites of anaerobic nitrate-dependent benzene oxidation by strain B.cereus were identified as phenol and benzoate.The results of substrate interaction between binary combinations for benzene,phenol and benzoate showed that the simultaneous presence of benzene stimulated the degradation of benzoate,whereas the addition of benzene inhibited the degradation of phenol.Benzene degradation by B.cereus was enhanced by the addition of phenol and benzoate,the enhanced effects were more pronounced at higher concentration.To our knowledge,this is the first report that the isolated bacterial culture of B.cereus can efficiently degraded benzene under nitrate reducing conditions.A pure culture using benzene as sole carbon and energy sources was isolated by screening procedure from gasoline contaminated soil.The analysis of the 16S rDNA gene sequence,morphological and physiological characteristics showed that the isolated strain was a member of genus Bacillus cereus.The biodegradation performance of benzene by B.cereus was evaluated,and the results showed that benzene could be efficiently biodegraded when the initial benzene concentration was below 150 mg/L.The metabolites of anaerobic nitrate-dependent benzene oxidation by strain B.cereus were identified as phenol and benzoate.The results of substrate interaction between binary combinations for benzene,phenol and benzoate showed that the simultaneous presence of benzene stimulated the degradation of benzoate,whereas the addition of benzene inhibited the degradation of phenol.Benzene degradation by B.cereus was enhanced by the addition of phenol and benzoate,the enhanced effects were more pronounced at higher concentration.To our knowledge,this is the first report that the isolated bacterial culture of B.cereus can efficiently degraded benzene under nitrate reducing conditions.

关 键 词：Bacillus cereus nitrate reduction anaerobic biodegradation substrate interaction PHENOL BENZOATE 

分 类 号：Q949.32[生物学—植物学] X52[环境科学与工程—环境工程]