Ultraviolet irradiation induced oxidative stress and response of antioxidant system in an intertidal macroalgae Corallina officinalis L.  

作　　者：Lixia Li Jiqiang Zhao Xuexi Tang 

机构地区：[1]College of Marine Life Science, Ocean University of China, Qingdao 266003, China [2]Chemistry and Biology College, Yan Tai University, Yantai 264005, China

出　　处：《Journal of Environmental Sciences》2010年第5期716-722,共7页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (No. 30270258);the Natural Science Foundation of Shandong Province (No.2007ZRB01903)

摘　　要：The response of the antioxidant defense system of an intertidal macroalgae Corallina officinalis L.to different dosages of UV-B irradiation was investigated.Results showed that superoxide dimutase （SOD） and peroxidase （POX） increased and then maintained at a relatively stable level when subjected to UV-B irradiation.Catalase （CAT） activity under medium dosage of UV-B irradiation （Muv） and high dosage of UV-B irradiation （Huv） treatments were significantly decreased.Ascorbate peroxidase （APX） activity first remained unaltered and then increased in Huv treatment.In addition,the assay on isozymes was carried out using non-denaturing polyacrylamide gel electrophoresis （PAGE）.The activities of some SOD isoforms were altered by UV-B.Two new bands （POX V and POX VII） appeared upon exposure to all three UV-B dosages.CAT III activity was increased by low dosage of UV-B irradiation （Luv）,whereas CAT III and CAT IV disappeared when the alga was exposed to Muv and Huv.Two bands of APX （APX VI and APX VII） were increased and a new band （APX X） was observed under Huv exposure.H 2 O 2 and thiobarbituric acid reacting substance （TBARS） increased under Muv and Huv treatments.Overall,UV-B protection mechanisms are partly inducible and to a certain extent sufficient to prevent the accumulation of damage in C.officinalis.The response of the antioxidant defense system of an intertidal macroalgae Corallina officinalis L.to different dosages of UV-B irradiation was investigated.Results showed that superoxide dimutase （SOD） and peroxidase （POX） increased and then maintained at a relatively stable level when subjected to UV-B irradiation.Catalase （CAT） activity under medium dosage of UV-B irradiation （Muv） and high dosage of UV-B irradiation （Huv） treatments were significantly decreased.Ascorbate peroxidase （APX） activity first remained unaltered and then increased in Huv treatment.In addition,the assay on isozymes was carried out using non-denaturing polyacrylamide gel electrophoresis （PAGE）.The activities of some SOD isoforms were altered by UV-B.Two new bands （POX V and POX VII） appeared upon exposure to all three UV-B dosages.CAT III activity was increased by low dosage of UV-B irradiation （Luv）,whereas CAT III and CAT IV disappeared when the alga was exposed to Muv and Huv.Two bands of APX （APX VI and APX VII） were increased and a new band （APX X） was observed under Huv exposure.H 2 O 2 and thiobarbituric acid reacting substance （TBARS） increased under Muv and Huv treatments.Overall,UV-B protection mechanisms are partly inducible and to a certain extent sufficient to prevent the accumulation of damage in C.officinalis.

关 键 词：UV-B irradiation Corallina officinalis L. superoxide dimutase CATALASE ascorbate peroxidase PEROXIDASE 

分 类 号：Q178.531[生物学—水生生物学] TS103.823[生物学—普通生物学]