应用光合色素标记物研究2001年2～6月厦门西海域浮游植物群落结构  

Phytoplankton community structure in western Xiamen waters from February to June 2001 from analysis of photosynthetic pigments

作　　者：何学佳[1] 高亚辉[2] 彭兴跃[2] 

机构地区：[1]暨南大学赤潮与水环境研究中心,广东广州510632 [2]厦门大学生命科学学院,福建厦门361005

出　　处：《台湾海峡》2010年第2期228-233,共6页Journal of Oceanography In Taiwan Strait

基　　金：国家自然科学基金资助项目(49806004);暨南大学博士启动基金资助项目(51208049);广东省自然科学基金资助项目(9451063201002243)

摘　　要：利用反相高效液相色谱(RP-HPLC)对厦门西海域2001年2～6月的浮游植物色素进行了测定,并首次运用CHEMTAX软件对该海域进行了浮游植物群落组成的分析,同时分析了环境因子对群落组成的作用.我们首先改进了RP-HPLC分离方案,分离的时间缩短了近1/3,达到了提高分离效率的目的.利用改进后的RP-HPLC方案进行色素分析显示,厦门西海域2001年2～6月期间,主要的特征色素是岩藻黄素(fucoxanthin)、19’-丁酰基氧化岩藻黄素(19’-butanoyloxyfucoxanthin)、19’-己酰基氧化岩藻黄素(19’-hexanoyloxyfucoxanthin)和多甲藻素(peridinin),月平均含量分别在267.824～655.076、20.118～126.764、65.693～214.115、26.955～203.927μg/m3的范围变动.CHEMTAX计算的结果进一步表明:硅藻是主要优势类群,其次是定鞭金藻和甲藻,分别占总生物量的36.14%～79.27%、4.50%～37.40%和4.83%～21.37%.总生物量从2月到6月呈增长趋势,温度是主要的决定因子.硅藻和定鞭金藻所占生物量的份额呈相反趋势变化,表明种间竞争也是重要的因素之一.Photosynthetic pigments were quantified,using RP-HPLC,from phytoplankton samples collected in western Xiamen waters from February to June in 2001.Diagnostic pigment data were converted into chlorophyll a biomass values to assess phytoplankton community structure using CHEMTAX software.The effects of environmental factors on phytoplankton community were also analyzed.An improved RP-HPLC protocol was employed which consistently reduced the separating time by a third.The separation of pigments using the improved RP-HPLC protocol suggested that the prime diagnostic pigments in this area during the investigation were Fucoxanthin,19＇-butanoyloxyfucoxanthin,19＇-hexanoyloxyfucoxanthin and peridinin with average monthly concentrations varying in the following ranges： 267.824～ 655.076,20.118～126.764,65.693～214.115 and 26.955～203.927μg/m3,respectively.CHEMTAX calculations demonstrated that the dominant group was diatoms with a contribution of 36.14% to 79.27% to total phytoplankton biomass,followed by prymnesiophytes and dinophytes,which accounted for 4.50%～37.40% and 4.83%～21.37%,respectively.Total phytoplankton biomass increased from February to June,in response to the continuously increasing temperature.The relative contribution of diatoms varied in a trend opposite to that of prymnesiophytes,suggesting that inter-species competition was an important factor for phytoplankton community structure in this area.

关 键 词：海洋生物学 特征色素 浮游植物群落结构 厦门西海域 

分 类 号：P735[天文地球—海洋生物学]