石油烃降解菌的分离鉴定及其产生乳化剂条件  

Isolation and Characterization of a Hydrocarbon-Degrading Bacterial Strain

作　　者：任华峰[1] 张雨山[1] 王静[1] 张晓青[1] 成玉[1] 

机构地区：[1]国家海洋局天津海水淡化与综合利用研究所,天津300192

出　　处：《化学工业与工程》2010年第3期189-194,共6页Chemical Industry and Engineering

基　　金：国家科技支撑项目(2006BAB03A08);中央级公益性科研院所基本科研业务费专项资金项目(K-JBYWF-2007-G13)

摘　　要：从天津塘沽原油污染海滩的泥样中分离得到一株石油烃降解菌SY095,经生理生化及16S rRNA基因序列分析鉴定为红球菌(Rhodococcus sp.)。该菌株能以正十六烷为碳源代谢产生一种对柴油等烃类具有良好乳化作用的生物乳化剂。菌株SY095产生乳化剂的最适宜条件为:正十六烷10g/L,初始pH值为7.2,30℃下160r/min摇床培养2d。在此条件下,培养液表面张力降到最低值,约32mN/m;经测定,其临界胶束浓度(CMC)值约为75mg/L。A bacterial strain SY095 was isolated from the sludge samples polluted by the municipal solid waste in the beach of Bohai sea. It was identified as Rhodococcus sp. according to its phenotype,physio-logical and chemical properties,and its 16S rRNA gene sequence. The results indicated that strain SY095 could produce bioemulsifier efficiently when n-hexadecane was used as carbon source. The opti- mal conditions for the synthesis of bioemulsifier were： 10 g /L of n-hexadecane,media initial pH 7. 2, cultivation was carried out for 2 d at 30 ℃ on a rotary shaker at 160 r /min. Under these conditions the surface tension of culture decreased to the lowest value,around 32 mN /m,after 2 d. The critical micelle concentration （CMC） of the bioemulsifier was 75 mg /L.

关 键 词：石油污染 石油烃降解 红球菌 生物乳化剂 

分 类 号：Q93[生物学—微生物学]