白术中多菌灵农药残留量分析  

Retention analysis of carbendazol in Atractylodes macrocephala

作　　者：王瑶[1] 富徐燕[1] 王霞[2] 白岩[1] 杨赛赛[1] 潘兰英[1] 田薇[1] 

机构地区：[1]浙江农林大学林业与生物技术学院,浙江临安311300 [2]山东省分析测试中心,山东济南250000

出　　处：《浙江师范大学学报（自然科学版）》2010年第2期189-192,共4页Journal of Zhejiang Normal University:Natural Sciences

基　　金：浙江省教育厅科研项目(20070947);浙江省高等教育研究会立项项目(Y200724)

摘　　要：建立了测定中药材白术中多菌灵农药残留量的方法:反相高效液相色谱法(RP-HPLC),色谱柱为ODS不锈钢柱,流动相为甲醇-水(体积比为35∶65),检测波长为281 nm.结果显示:多菌灵在0.45～108 ng进样量与峰面积有良好的线性关系,最低检出量为3.6×10-10g,最低检测质量浓度为0.022 5μg/mL.该方法简便、快速,适合同类中药材中多菌灵农药残留量的测定.It was aimed to establish an HPLC method for the determination of carbendazol in Chinese Traditional Medicine Atractylodes macrocephala.The mobile phase constituted of methanol-water（35 ∶ 65）.A C-18 column was used,and carbendazol was determined at the detection wavelength of 281 nm.In the linear range of 0.45～108 ng,the sample size and the peak area of carbendazol showed a good linear relationship,the limit of detection was 3.6×10＋-10 g,the minimum detectable concentration was 0.022 5 μg/mL.The method was simple,rapid,reliable,suitable for the determination and safe control of residual carbendazol in this kind of Chinese Traditional Medicine.

关 键 词：白术 多菌灵 高效液相色谱 农药残留量 

分 类 号：O657.7[理学—分析化学]