合成聚-β-羟基丁酸芽孢杆菌的筛选、鉴定及碳氮源优化  

Screening,identification and optimization of carbon,nitrogen sources of bacillus producing poly-β-hydroxybutyrate

作　　者：周琴[1] 蒋冬花[1] 杨叶[1] 嵇豪[1] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004

出　　处：《浙江师范大学学报（自然科学版）》2010年第2期203-209,共7页Journal of Zhejiang Normal University:Natural Sciences

基　　金：浙江省重大科技专项(2007C12036);浙江省自然科学基金资助项目(Y3090343)

摘　　要：从污泥中分离纯化,筛选得到一株高产聚-β-羟基丁酸的菌株,标号为Bm-10,针对不同碳氮源对Bm-10产聚-β-羟基丁酸的影响进行了研究,结果表明最适碳氮源分别为葡萄糖和牛肉膏.菌株Bm-10经形态、生理生化和16S rDNA基因序列鉴定,确定为巨大芽孢杆菌(Bacillus megaterium).A high-yield poly-β-hydroxybutyrate strain Bm-10 was screened from sludge.The effects on poly-β-hydroxybutyrate yielding with different carbon and nitrogen sources were studied.The results showed,the optimal carbon source was glucose,and the best nitrogen source was beef paste.Strain Bm-10 was identified as Bacillus megaterium by morphological observation,physiological and biochemical test,and 16S rDNA sequence analysis.

关 键 词：聚-Β-羟基丁酸 碳源 氮源 芽孢杆菌 

分 类 号：Q93[生物学—微生物学]