TEM型β内酰胺酶新亚型TEM-166编码基因的克隆表达  

Cloning of TEM-166 β-lactamases gene

作　　者：王慧[1,2] 熊自忠[1] 李睿[1] 

机构地区：[1]安徽医科大学第一附属医院感染病科,合肥230022 [2]安庆市第一人民医院传染病科

出　　处：《中国感染与化疗杂志》2010年第2期120-122,共3页Chinese Journal of Infection and Chemotherapy

基　　金：教育部科学技术研究重点项目(209059);安徽省优秀青年科技基金(08040106815);安徽省人事厅基金资助项目;卫生厅人才基金资助项目

摘　　要：目的了解临床分离大肠埃希菌Ayfy2926中TEM型β内酰胺酶的基因型。方法采用聚合酶链反应(PCR)扩增大肠埃希菌Ayfy2926中TEM型β内酰胺酶的全编码基因并对PCR产物进行序列分析;PCR产物克隆入pHSG398质粒并在感受态细胞大肠埃希菌JM109中进行表达,琼脂稀释法测定转化子菌株对β内酰胺类抗菌药的敏感性。结果大肠埃希菌Ayfy2926中TEM型β内酰胺酶的基因型为一种新亚型,TEM-166(GenBank注册号FJ197316);与TEM-1相比,仅有1个氨基酸差异(Arg120→Gly);与TEM-1转化子菌株相同,TEM-166转化子菌株对哌拉西林高度耐药,对第三代头孢菌素、亚胺培南敏感,β内酰胺酶抑制剂可显著降低克隆表达菌株的耐药性。结论TEM-166为TEM型β内酰胺酶的新亚型,是一种非超广谱β内酰胺酶。Objective To investigate the TEM β-lactamases gene in the isolate of Escherichia coli Ayfy2926.Methods The TEM β-lactamase gene was amplified by PCR and sequenced.The purified PCR product was cloned into pHSG398 vector and transformed into E.coli JM109.The antibiotic susceptibility of transformant was tested by agar dilution method.Results The TEM β-lactamases gene was identified as a new subtype,TEM-166,with 1 amino acid substitution between TEM-1 and TEM-166（GenBank accession no.FJ197316）.The transformant with TEM-166 gene was resistant to piperacillin and susceptible to the third generation cephalosporins,imipenem and piperacillin-clavulanic acid.Conclusions TEM-166 is a new subtype of TEM β-lactamase,which is belonged to non-extended spectrum β-lactamase.

关 键 词：大肠埃希菌 TEM型 Β内酰胺酶 TEM-166 

分 类 号：R450[医药卫生—治疗学]