选择性亚低温对脑外伤大鼠脑组织Fas-L基因表达的影响  

Effects of Selective Mild Hypothermia on Expression of Fas ligand mRNA in Rats After Traumatic Brain Injury

作　　者：江素君[1] 袁红[1] 张银霞[2] 梅依婷[2] 裘五四[2] 

机构地区：[1]杭州师范大学医学实验中心,浙江杭州310036 [2]杭州师范大学临床医学院,浙江杭州310036

出　　处：《健康研究》2010年第2期92-94,共3页Health Research

基　　金：杭州市医药卫生科技计划项目(2007A011)

摘　　要：目的研究选择性亚低温治疗大鼠脑外伤时对脑组织Fas-L基因表达的影响。方法 72只大鼠随机分为假手术组、亚低温组和常温对照组。采用改进的Feeney自由落体法制作重型外伤性颅脑损伤模型,局部降温法在伤后30min内达到亚低温目标并保持6h,RT-PCR检测3组大鼠伤后不同时间点脑组织Fas-L基因表达。结果亚低温组大鼠在损伤后不同时间点(6h,24h,48h和72h)脑组织的Fas-LmRNA的相对含量分别为0.54±0.07、0.47±0.06、0.41±0.06、0.67±0.08,常温对照组分别为1.53±0.21、0.91±0.11、1.33±0.15、1.56±0.171.53,相同时间点两组间比较均有显著性差异(P<0.05)。结论亚低温对大鼠外伤性颅脑损伤急性期具有降低Fas-LmRNA的作用。Objective To study the effects of local mild hypothermia on Fas ligand （Fas-L） mRNA in rats after traumatic brain injury. Methods Seventy-two Sprague-Dawley rats were randomly divided into sham-operation group, mild hypothermia group and normothermia group. Feeney＇ s model was used to produce traumatic brain injury. Local therapeutic hypothermia was achieved 30 minutes after injury by surface cooling and maintained for 6 hours. The expression of brain Fas- L mRNA was detected by reverse transcription polymerase chain reaction （RT-PCR）. Results The expression of rat brain Fas-L mRNA in the mild hypothermia group was much lower than that in the normothermia group at 6 hours, 24 hours after injury （ 0.54, 0.47, 0.41 and 0.67 versus 1. 53, 0. 917, 1. 33 and 1. 56, respectively, P 〈0.05）. Conclusion Mild hypothermia can down-regulate the Fas-L expression of brain tissue in the acute phase of traumatic brain injury.

关 键 词：选择性亚低温 脑损伤 脑水肿 Fas—L基因 大鼠 

分 类 号：R651.1[医药卫生—外科学]