“台农17号”菠萝组培快繁技术研究  

Tissue culture and rapid propagation technology for pineapple variety Tainong 17

作　　者：张瑛[1] 刘业强[2] 苏伟强[2] 

机构地区：[1]广西农业科学院葡萄与葡萄酒研究所,南宁530007 [2]广西农业科学院园艺研究所,南宁530007

出　　处：《广西农业科学》2010年第4期310-312,共3页Guangxi Agricultural Sciences

基　　金：农业部行业科技专项项目(nyhyzx07-030);广西科技攻关项目(桂科攻0719003-3);广西自然科学基金项目(桂科自0728068)

摘　　要：以菠萝品种"台农17号"冠芽为外植体,采用1.0～5.0 mg/L 6-BA、0.1～0.5 mg/L NAA、1.0～4.0 mg/L IBA激素配比对芽诱导、继代增殖和生根进行培养。结果表明,适宜菠萝冠芽诱导和继代增殖培养的培养基为MS+6-BA 3.0mg/L+NAA 0.1 mg/L,继代增殖周期在22 d较为适宜,增殖系数为6.8;适宜生根培养基为1/2MS+IBA 3.0 mg/L+NAA 0.5mg/L,以沙∶塘泥(1∶2)为假植基质,假植成活率达94.0%,植株生长健壮,长势良好。The crown bud of pineapple variety Tainong 17 was used as explants, different hormone combinations rang- ing from 1.0-4.0 mg/L 6-BA, 0.1-0.5 mg/L NAA and 1.0-4.0 mg/L IBA were added to MS or 1/2MS medium to conduct the induction of bud, subculture of cluster buds and rooting of plantlets. The results showed that MS＋6-BA （3.0 mg/L）＋ NAA（0.1 mg/L） was the best medium for the induction of crown buds, and subculture of the cluster buds with 6.8 of mul- tiplication coefficient in period of 22 days. The optimal medium for rooting was 1/2MS ＋ IBA（3.0 mg/L）＋ NAA（0.5 mg/L）, and the mixture of sand and pond sludge （1：2） was found to be the best temporary planting medium for rooted plantlets with 94% of survival rate, and the plantlets grew well and vigorouly.

关 键 词：菠萝 台农17号 冠芽 诱导 增殖 快繁 

分 类 号：S668.303.53[农业科学—果树学]