急性纯氧对SD大鼠损伤的研究  

Studies on the injury of SD rats exposed to 100% oxygen

作　　者：谢仰民[1] 符乃阳[2] 张尔贤[2] 

机构地区：[1]汕头大学医学院实验动物中心,汕头515031 [2]汕头大学理学院生物学系,汕头515063

出　　处：《汕头大学学报（自然科学版）》1998年第1期20-24,共5页Journal of Shantou University：Natural Science Edition

摘　　要：SD大鼠暴露于1ATA100％的急性纯氧中，对其血细胞和肺脏进行研究，以阐明急性纯氧对机体的损伤机制．结果表明：SD大鼠暴露于100％急性纯氧中不同时间，血液中红细胞的浓度（RBC），红细胞压积（HCT），血红蛋白浓度（HGB），平均红细胞血红蛋白含量（MCH），平均红细胞体积（MCV），平均红细胞血红蛋白浓度（MCHC），白细胞浓度（WBC）等无显著变化（P＞0．05）；但是，白细胞的分类变化显著（P＜0．05）或非常显著（P＜0.01或P＜0．001），淋巴细胞随SD大鼠在急性纯氧中暴露的时间延长不断下降，中性粒细胞和单核细胞的总数不断升高．急性氧中暴露65小时后，20％的大鼠死亡，存活的大鼠解剖，发现肺部出现严重的炎症，肺的湿重．脂质过氧化（LPO）产物显著增加（P＜0．001），超氧化物歧化酶（SOD）活力明显下降（P＜0．001）．In order to elucidate the injury mechanism of SD rats by 100% oxygen, blood cells and lungs of SD rats exposed to 100% oxygen were studied. Between the control rats and SD rats exposed to 100% oxygen for different time periods, no significant differences were observed in red blood cell concentration hematocrit (HCT), mean cell volume (MCV), mean cell hemoglobin (MCH), mean cell hemoglobin concentration (MCHC),and white blood cell concentration (P > 0. 05 ). However, differential WBC count changed significantly (P <0. 05) or very significantly (0. 001< P <0. 01, or P <0. 001 ), lymphocytes (LYMPH) decreased monocytes and granulocytes (MO+GR) increased gradually. After 65 hours, 2096 of SD rats were dead. When the survivors were dissected, it was found that all of them got inflammation in their lungs, both the moist weight of their lungs and the products of lipid peroxidation (LPO) increased significantly (P <0. 001 ), and the activity of their superoxide dismutase (SOD) decreased very significantly (P <0. 001 ).

关 键 词：急性 纯氧 氧自由基 LPO SOD 机体损伤 

分 类 号：R459.6[医药卫生—治疗学]