间充质干细胞株对小鼠骨髓来源树突状细胞分化成熟的影响  

Effects of Mesenchymal Stem Cell Line on Differentiation and Maturation of Murine Bone Marrow-derived Dendritic Cells

作　　者：李毅平[1] 王泳[1] 许云云[1] 翁震[1] 袁雅红[1] 张学光[1] 

机构地区：[1]苏州大学医学生物技术研究所,江苏苏州215007

出　　处：《苏州大学学报（医学版）》2010年第1期7-10,49,F0002,共6页Suzhou University Journal of Medical Science

基　　金：国家自然科学基金资助项目(No.30400395);国家教育部留学回国人员科研启动基金资助项目(K5120506)

摘　　要：目的探讨间充质干细胞株C3H10T1/2对小鼠骨髓来源树突状细胞(dendritic cells,DCs)体外分化、成熟的影响。方法采用密度梯度离心法分离小鼠骨髓细胞,在mGM-CSF和mIL-4刺激下获得大量未成熟DCs。将DCs和C3H10T1/2细胞共培养2d,同时加入LPS刺激,分别通过倒置显微镜、流式细胞仪,混合淋巴反应以及ELISA检测DCs的成熟。结果经C3H10T1/2细胞体外共培养的DCs,形态观察呈散在分布,细胞边缘圆滑,流式细胞仪检测显示其低表达CD11c、MHC-Ⅱ、CD80、CD86、CD40;共培养组的DCs刺激同种异型小鼠脾细胞增殖的能力在各个浓度均明显低于对照组(P<0.05或0.01);ELISA检测培养上清中IFN-γ和IL-10的浓度也明显低于对照组(P<0.05或0.01)。结论间充质干细胞株C3H10T1/2可以明显抑制小鼠骨髓来源DCs的体外成熟。Objective To study on the biological characteristics of murine bone marrow-derived dendritic cells（DCs）regulated by mesenchymal stem cell line C3H10T1/2 in vitro.Methods Murine bone marrow cells were isolated by density gradient centrifugation and cultured with mGM-CSF and mIL-4.Large amounts of immature DCs（iDCs）were obtained.Then DCs were co-cultured with C3H10T1/2 cells in the presence of LPS for 2 days.The effects of C3H10T1/2 cells on the maturation of DCs were analyzed with inverted microscope,flow cytometry,mixed lymphocyte reaction and ELISA.Results DCs co-cultured with C3H10T1/2 cells were scattered and round observed under inverted microscope.FACS analyses showed that those DCs expressed low levels of CD11c,MHC-Ⅱ,CD80,CD86 and CD40.Allogeneic spleenocytes proliferation assay showed that the stimulatory ability of DCs from co-culture group was significantly lower than that in the control group.The IFN-γ and IL-10 secretion in the culture supernants were lower in the co-culture group compared with that in the control group.Conclusion Mesenchymal stem cell line C3H10T1/2 can significantly suppress the maturation of murine bone marrow derived DCs in vitro.

关 键 词：树突状细胞 间充质干细胞 C3H10T1/2 体外成熟 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学]