凝血酶受体在多发性骨髓瘤细胞XG-1增殖和抗凋亡作用中的机制  

Study on the Role of Thrombin Receptor in the Proliferation and Apoptosis of Multiple Myeloma Cell Line,XG-1

作　　者：朱蕴兰[1] 李爽[1] 于丰源[1] 杨荣秉[1] 张秀春[1] 徐晓军[1] 王月丹[1] 

机构地区：[1]北京大学基础医学院免疫学系,北京100191

出　　处：《苏州大学学报（医学版）》2010年第1期15-17,41,共4页Suzhou University Journal of Medical Science

基　　金：国家自然科学基金资助项目(30771995);北京大学创新人才支持计划资助项目(30671962)

摘　　要：目的研究凝血酶及其受体在促进多发性骨髓瘤细胞增殖和抗凋亡作用中的机制。方法采用免疫荧光标记、流式细胞仪检测、细胞计数和免疫印迹等方法,对多发性骨髓瘤细胞株XG-1细胞表达的凝血酶受体、凝血酶对XG-1的增殖与抗凋亡作用及细胞内JAK2-STAT3分子磷酸化进行分析。结果凝血酶可以通过激活XG-1表面的凝血酶受体,继而激活JAK2-STAT3途径的磷酸化,促进骨髓瘤细胞的增殖与抗凋亡作用,且该作用与gp130介导的信号转导作用无关。结论凝血酶及其受体介导的信号转导通路是多发性骨髓瘤维持增殖和抗凋亡作用的因素之一。Objective To study the roles of thrombin and its receptor in the proliferation and ant-apoptosis multiple myeloma（MM）cells.Methods The effects of thrombin on the thrombin receptor expression,proliferation and apoptosis of XG-1,a MM cell line were evaluated by using immunofluorescence analysis,flow cytometery,cell counting,and western blot,respectively.Results Thrombin induced cell proliferation and provented apoptosis in XG-1 cells were founded through JAK2-STAT3 phosphorylation pathway which was IL-6 independent.Conclusion Thrombin and its receptor inducing signal transduction pathway is one of the effective factors in the proliferation and survival of MM cells.

关 键 词：多发性骨髓瘤 白细胞介素-6 凝血酶 GP130 

分 类 号：R392.12[医药卫生—免疫学]