大鼠脑内移植超顺磁性氧化铁标记骨髓基质干细胞的MRI扫描序列优化研究  

Optimization of MRI Sequences in Observation of SPIO-labled Bone Marrow Stromal Cells Transplanted into Rat Brain

作　　者：郭静[1] 王兰[2] 张荣俊[3] 罗蔚锋[2] 贡志刚[3] 孙敬[1] 徐菡[1] 沈钧康[1] 

机构地区：[1]苏州大学附属第二医院影像科,江苏苏州215004 [2]苏州大学附属第二医院神经内科,江苏苏州215004 [3]苏州大学附属第二医院神经外科,江苏苏州215004

出　　处：《苏州大学学报（医学版）》2010年第1期31-33,F0003,共4页Suzhou University Journal of Medical Science

基　　金：江苏省高校自然科学基金资助项目(06KJB320097)

摘　　要：目的筛选并确定大鼠纹状体定向移植超顺磁性氧化铁(SPIO)标记骨髓基质干细胞(BMSCs)进行MRI活体观察的最佳扫描序列。方法32只大鼠于右侧纹状体单点注入20μlSPIO标记的BMSCs。使用1.5T超导磁共振成像仪和显微线圈,运用TSE-T1WI、TSE-T2WI和FFE-T2WI3种序列在大鼠移植细胞后即刻行MRI检查,测量并比较各序列图像的面积及信号强度,筛选出最佳观察序列。结果注射SPIO标记BMSCs的大鼠MRI检查于各序列断面图上纹状体区均可见类圆形、不规则形低信号区。其中FFE-T2WI序列显示低信号区域最大,且平均信号强度最小;TSE-T1WI序列低信号区域最小,平均信号强度最大;TSE-T2WI序列居中。结论使用1.5T超导磁共振成像仪和显微线圈,TSE-T1WI、TSE-T2WI和FFE-T2WI3种序列中FFE-T2WI序列可作为SPIO标记BMSCs进行MRI活体示踪最敏感的序列。Objective To determine the optimal MRI sequence in observation of superparamagnetic iron oxide（SPIO）-labled bone marrow stromal cells（BMSCs）transplanted into rat brain.Methods SPIO-labled BMSCs were transplanted into the brain of 32 rats.TSE-T1WI、TSE-T2WI and FFE-T2WI were obtained immediately after transplantation to measure and the area and signal intensity of hypointense areas of different sequences with 1.5 T 47 mm inner diameter micro-coil compared.Results Round or irregular hypointense areas were observed in the brain of all the rats which were transplanted with SPIO-labled BMSCs in the images of the three sequences.Among these,FFE-T2WI showed the biggest hypointense area and the minimum signal intensity.Conclusion FFE-T2WI sequence is the most sensitive sequence to observe SPIO-labled BMSCs with 1.5 T 47 mm inner diameter micro-coil in vivo.

关 键 词：骨髓基质干细胞 超顺磁性氧化铁 磁共振成像 序列 

分 类 号：R445.2[医药卫生—影像医学与核医学]