脂质体包封血红蛋白(LEH)的制备及其对红细胞聚集性的影响  

PREPARATON OF LIPOSOMES ENCAPSULATED HEMOGLOBIN(LEH) AND ITS EFFECT ON ERYTHROCYTE GATHERING PROPERTY IN VITRO

作　　者：王怡[1] 翁维良[1] 刘剑刚[1] 李白霞[2] 孙曹民[2] 邓百鸣 

机构地区：[1]中国中医研究院西苑医院 [2]北京理工大学材料中心

出　　处：《中国生物医学工程学报》1998年第4期338-341,共4页Chinese Journal of Biomedical Engineering

基　　金：国家自然科学基金

摘　　要：本文报告了脂质体包封血红蛋白（Ｌｉｐｓｏｍｅ－ＥｎｃａｐｓｕｌａｔｅｄＨｅｍｏｇｌｏｂｉｎ，ＬＥＨ）制备方法，制备了以磷脂酸（ＰＡ）、磷脂酰丝氨酸（ＰＳ）分别作膜组分的两种ＬＥＨ，即ＬＥＨ（ＰＡ）和ＬＥＨ（ＰＳ）。应用准弹性光散射电泳（ＱＥＬＳ）仪测定其电泳率，发现ＬＥＨ（ＰＡ）电泳率明显快于ＬＥＨ（ＰＳ）。进一步观察两种ＬＥＨ体外对红细胞电泳率和红细胞聚集性影响。发现ＬＥＨ（ＰＡ）可降低红细胞聚集性（Ｐ＜０．０５）；Method of preparation of tiposomes encapsuated hemoglobin (LEH) was reported and two kinds of LEH prepared. Electrophoresis parameters of LEH were studied by quasi elasticity light scattering (QELS) technique. The results showed that the electrophoresis parameters of LEH(PA) was significantly higher than LEH(PS); the former taking phospholipid acid and the latter, phosphatidyl serine as composition of membrane. The effects of the two LEHs on erythrocyte electrophoretic parameters and erythrocyte gathering property were also studied in vitro, and results showed that LEH(PA)could decrease erythrocyte gathering property and increased RBC electrophoretic parameters 1 hr after LEH addition.

关 键 词：脂质体 血红蛋白 红细胞聚集 制备 

分 类 号：R944.9[医药卫生—药剂学] R977.8[医药卫生—药学]