RECK在子宫内膜异位症中的表达及其与MMP-9和VEGF的关系  

Expression of RECK gene in endometriosis and the relationship between MMP-9 and VEGF

作　　者：徐云钊[1] 张玉泉[1] 糜媛媛[1] 

机构地区：[1]南通大学附属医院妇产科,江苏226001

出　　处：《交通医学》2010年第2期125-128,共4页Medical Journal of Communications

摘　　要：目的:探讨RECK基因在子宫内膜异位症(EM)患者在位内膜和异位病灶中的表达及其与血管生成、组织浸润转移因子的关系。方法:采用半定量逆转录聚合酶链反应(RT-PCR)检测异位内膜40份,在位内膜24份和正常内膜20份中RECK、MMP-9和VEGF的表达。结果:异位病灶中RECK mRNA的相对表达水平较正常内膜和EM患者的在位内膜均降低(P<0.05);RECK mRNA在Ⅰ、Ⅱ期中的相对表达水平较Ⅲ、Ⅳ期显著降低(P<0.05);MMP-9 mRNA、VEGF mRNA在3组内膜组织之间相对表达量相比差异均有统计学意义。结论:RECK基因在EM中表达减少,而MMP-9 mRNA、VEGF mRNA在EMs中表达升高。RECK基因的表达减少或缺失可能为EM的发生发展机制之一,RECK基因的表达可能起到抑制MMP-9 mRNA、VEGF mRNA表达的作用。Objective：To investigate the expression of RECK gene in the eutopic endometrium and in the ectopic endometrium of women with endometriosis.To find the relationship of RECK gene and factor in vascularization and tissue infiltration.Method：The method of RT-PCR was used to detect the expression of RECK,MMP-9 and VEGF in 40 patients with 40 specimens of the ectopic endometrium and 24 specimens of the eutopic endometrium and 20 specimens of normal endometrium.Results：The relative experssion level of RECK mRNA in the ectopic endometrium was lower than that in the normal endometrium and the eutopic endometrium（P 0.05）.The relative expression level of RECK mRNA in stageⅠ、Ⅱ was conspicuously higher than that in stage Ⅲ and Ⅳ（P0.05）.The relative expression level of MMP-9 and VEGF mRNA in the three endometrium specimens were obviously different.Conclusions：The expression of RRCK decreased in endometriosis,but the expression of MMP-9 and VEGF mRNA was increased.It is possible that the decrease or loss of RECK gene is one of the mechanisms on the development and progression of endometriosis.The expression of RECK gene may depress the expression of MMP-9 and VEGF mRNA.

关 键 词：子宫内膜异位症 RECK基因 基质金属蛋白酶 血管内皮生长因子 逆转录聚合酶链反应 

分 类 号：R711.74[医药卫生—妇产科学]