国家种质库保存大豆和菜豆种质的种传病毒检测  

Detection of Seed-Borne Viruses in Soybean and Common Bean Seeds Conserved in the National Crop Genebank

作　　者：韩俊丽[1,2] 郭庆元[1] 杨知还[2] 张煜[2,3] 王晓鸣[2] 

机构地区：[1]新疆农业大学,乌鲁木齐830052 [2]中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程,北京100081 [3]河南农业大学,郑州450002

出　　处：《植物遗传资源学报》2010年第3期284-289,共6页Journal of Plant Genetic Resources

基　　金：国家科技支撑计划(2006BDAD13B10-02)

摘　　要：以国家种质中期库提供的300份大豆、100份菜豆种质为材料,分别采用血清学和分子生物学方法,对种传病毒的种类进行了检测。结果表明:在大豆种质中检测出大豆花叶病毒(SMV)、黄瓜花叶病毒(CMV)、苜蓿花叶病毒(AMV)3种病毒,阳性检出率分别为25.33%(76份)、13.67%(41份)和4.67%(14份)。大豆种质中还存在大豆花叶病毒与黄瓜花叶病毒、大豆花叶病毒与苜蓿花叶病毒的复合侵染。在菜豆种质中检测出菜豆普通花叶病毒(BCMV)阳性材料92份,种质带毒率高达92%。这些信息将会对今后采取相关措施提高国家种质库保存的豆类种质的质量提供帮助。In this study,300 soybean and 100 common bean germplasm conserved in National Crop Genebank were screened for seed-borne viruses by serological and molecular biological methods.For the soybean germplasms,76（25.33%）,41（13.67%）and 14（4.67%）were positive to soybean mosaic virus（SMV）,cucumber mosaic virus（CMV）,and alfalfa mosaic virus（AMV）respectively.Some were infected by two viruses,such as SMV and CMV,or SMV and AMV.In common bean 92% were infected by bean common mosaic virus（BCMV）.These information will be useful to understand the status of seed-borne viruses in leguminous crops in genebank and to take measures for control virus diseases during seed reproductions in field.

关 键 词：大豆 菜豆 种质 种传病毒 检测 

分 类 号：S565.1[农业科学—作物学] S643.1