检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
机构地区:[1]上海交通大学附属第一人民医院呼吸科,上海200080 [2]上海第二军医大学附属长海医院呼吸科,上海200433
出 处:《世界临床药物》2010年第5期276-279,共4页World Clinical Drug
摘 要:目的探讨真菌蛋白酶对人支气管上皮细胞系BEP2D细胞分泌细胞因子的影响。方法分别用0、1、2和4μmol/mL的真菌蛋白酶刺激人支气管上皮细胞系BEP2D细胞,共同培养6和12 h后收集细胞,用免疫印迹(western blotting)方法检测内皮细胞表达人胸腺基质淋巴细胞生成素(TSLP)蛋白水平,用定量PCR方法检测胸腺活化调节因子(TARC)、白介素(IL)-12和α肿瘤坏死因子(TNF-α)的mRNA表达。结果人支气管上皮细胞系BEP2D细胞TSLP蛋白及TARCmRNA表达增加,并呈剂量依赖关系,但IL-12和TNF-α表达水平无显著改变。结论真菌蛋白酶能刺激支气管上皮细胞BEP2D分泌与哮喘相关的细胞因子,其可能是真菌诱发哮喘的机制之一。Objective To observe the effect of fungus protease on the cytokines production of human bronchus epithelium cell line BEP2D. Methods Fungus proteases (0, 1, 2 and 4 μmol/mL) stimulated human bronchus epithelium cell line BEP2D and cultured cells were collected after 6 and 12 h. The level of TSLP protein was detected by western blotting, mRNA of cytokines (TARC, IL-12 and TNF-α) was observed by real-time PCR. Results The level of TSLP protein and mRNA of TARC was increased dose-dependently. But there was no significant change in the level of IL-12 or TNF-α. Conclusion Fungus protease can induce the expression of cytokines associated with asthma in bronchus epithelium cell line BEP2D, which may be one of the asthma pathogenesis by fungus protease.
分 类 号:R318.13[医药卫生—生物医学工程] R562.25[医药卫生—基础医学]
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.171