大熊猫核糖体蛋白L34基因cDNA克隆与蛋白特性  

cDNA Cloning of Ribosomal Protein L34 Gene from Giant Panda and Analysis to Features of the Encoding Protein

作　　者：杜玉杰[1] 侯怡铃[2] 侯万儒[2] 

机构地区：[1]湛江教育学院生化系,广东湛江524000 [2]西华师范大学生命科学学院,四川南充637002

出　　处：《重庆师范大学学报（自然科学版）》2010年第3期19-22,共4页Journal of Chongqing Normal University:Natural Science

基　　金：国家自然科学基金项目(No.30470261);四川省应用技术项目(No.2006J13-057);四川省教育厅重点科研项目(No.07ZA120)

摘　　要：为进一步研究RPL34的结构基因,也为更深入开展大熊猫分子生物学研究积累科学资料,本研究采用RT-PCR方法,首次成功克隆了大熊猫的核糖体蛋白L34基因的cDNA序列,对克隆序列进行了测序及初步分析,并利用RPL34蛋白构建系统树。结果表明:大熊猫RPL34基因的表达序列全长为379 bp,ORF为354 bp,编码117个氨基酸的蛋白质,该蛋白分子量为13.292 9 kD,等电点(pI)为11.48,含有5种类型共11个功能位点。进一步分析发现,该基因与已报道的人、牛、褐家鼠、小家鼠、非洲爪蟾和斑马鱼等物种的编码序列及其编码的氨基酸序列同源性很高,蛋白质分子量、pI非常接近,功能位点基本相同,说明核糖体蛋白L34基因及其编码蛋白在进化过程中非常保守;进化树分类结果与传统分类一致,表明L34蛋白能很好的反映物种间的进化关系。In order to study the structural gene of RPL34 and accumulate a wealth of data t＇or research on the molecular level, the cDNA of RPL34 was cloned successfully for the first time from the giant panda （Ailuropoda melanoleuca） using RT-PCR, which was also sequenced, analyzed preliminarily and constructed the tree using protein RPL34. The result shows that the cDNA fragment cloned is 379bp in size containing an open reading frame of 354 bp. The deduced protein sequence shows that the protein is composed of 117amino acids and its estimated molecular weight is 13. 292 9 kD with a pI of 11.48. Topology prediction shows there are eleven functional sites clarified five types. Alignment analysis indicates that the nucleotide sequence and the deduced amino acid sequence share a high homology with some species studied, including Homo sapiens, Bos Taurus, Rattus norvegicus, Mus musculus, Xenopus laevis and Danio rerio. The molecular weight and pI are more closed and functional sites are roughly the same. These results display the gene and its encoding protein is highly conserved. The same category between the tree constructed by Ribosomal Protein L34 and the traditional classify indicate that the RPL34 can reflect the relationship among species very well.

关 键 词：大熊猫 RT—PCR RPL34 克隆 分析 

分 类 号：Q959.838[生物学—动物学] Q343.17