还脑益聪方对早期认知功能障碍大鼠海马病理形态、炎症因子和氧化应激指标的影响  

Effect of Huannao Yicong Formula on Pathomorphology,Inflammatory Factor and Oxidative Stress Index of Hippocampus in Cognitive Impairment Rats at Early Stage

作　　者：姚明江[1] 李浩[1] 赵文明[1] 刘剑刚[1] 徐立[1] 官杰[1] 蔡琳琳[1] 

机构地区：[1]中国中医科学院西苑医院,北京100091

出　　处：《世界中西医结合杂志》2010年第4期300-304,共5页World Journal of Integrated Traditional and Western Medicine

基　　金：国家自然基金项目(No.30672571);国家自然基金项目(No.30873338);国家中医药管理局科技专项资助项目(No.04-05JP65)

摘　　要：目的观察还脑益聪方对复合因素所致早期认知功能障碍大鼠行为学及海马病理形态、炎症因子和氧化应激指标的影响。方法采用皮下注射D-半乳糖及饲喂半高脂饲料的方法造成早期认知功能障碍大鼠模型,并以还脑益聪方灌胃治疗。采用Morris水迷宫试验观察大鼠行为学变化,HE染色及透射电镜观察海马病理形态学改变;应用放射免疫法检测大鼠海马匀浆白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)含量,酶联免疫法(ELISA法)测定氧化低密度脂蛋白(ox-LDL)含量,免疫比色法测定丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX)和谷胱甘肽S转移酶(GSH-ST)含量。结果还脑益聪方能增加模型大鼠水迷宫试验穿台次数(P<0.01),减轻模型大鼠海马神经细胞损伤、线粒体病变及胶质细胞增生,降低海马匀浆TNF-α及ox-LDL含量(P<0.05,P<0.01)。结论还脑益聪方能提高早期认知功能障碍模型大鼠的空间学习记忆能力,这可能与其抑制脑内炎症反应与氧化应激,从而减轻海马组织病理变化有关。Objective To observe the effect of huannao yicong formula on pathomorphology,inflammatory factor and oxidation stress index of hippocampus in cognitive impairment rats at early stage induced by compound factors.Methods The cognitive impairment rat models were induced by hypodermic D-galactose injection and half fat feeder,and were treated with huannao yicong formula via intragastric administration.Morris water maze was used to observe the praxiological changes of rats.HE staining and transmission electron microscope（TEM）were used to review the pathomorphological changes of hippocampus;the radioimmunoassy was used to measure the contents of IL-6 and TNF-α in hippocampal homogenate.ELISA method was used to detect the level of ox-LDL and colorimetric method was used to detect the contents of MDA,GSH-PX and GSH-ST.Results Huannao yicong formula increased the frequency of swimming through the platform of model rats（P〈0.01）,alleviated neuron impairment,mitochondrial damage and proliferation of glial cells,decreased the contents of TNF-α and ox-LDL in hippocampal homogenate（P〈0.05,P〈0.01）.Conclusion Huannao yicong formula can improve the spatial learning and memory ability of cognitive impairment rats at early stage,which may be related to its effects on inhibiting inflammatory reaction and oxidative stress,and thus alleviating the pathomorphological changes in hippocampus tissue.

关 键 词：还脑益聪方 认知功能障碍 行为学 病理 炎症 氧化应激 

分 类 号：R543.5[医药卫生—心血管疾病]