一株天鹅源H5N1亚型禽流感病毒的分离鉴定  

Identification of H5N1 Subtype Avian Influenza Virus From Swan

作　　者：郑煦灿[1] 焦培荣[1] 单芬[1] 韦艳梅[1] 乔立旺[1] 李芳[1] 韦良孟[1] 辛朝安[1] 任涛[1] 廖明[1] 罗开健[1] 

机构地区：[1]华南农业大学兽医学院农业部动物疫病防控重点开放实验室广东省动物源性人兽共患病预防与控制重点实验室,广东广州510642

出　　处：《动物医学进展》2010年第5期5-8,共4页Progress In Veterinary Medicine

基　　金：教育部新世纪优秀人才支持计划项目(NCET-06-0752);教育部创新团队项目(IRT0723);国家肉鸡产业技术体系项目(nycytx-42-G3-03);广东省自然科学基金项目(8451064201001132);广东省科技计划项目(2007A020300011)

摘　　要：2004年从广东某地送检的病死天鹅肺组织中分离到1株禽流感病毒,用血凝抑制试验(HI)、聚合酶链反应(PCR)、基因测序等对其进行了亚型鉴定。结果表明,此分离株具有血凝活性,且H5亚型禽流感病毒标准阳性血清能对其产生特异性抑制作用;分别用针对禽流感病毒M基因、H5亚型禽流感病毒HA基因、N1亚型禽流感病毒NA基因特异性引物对该分离株进行PCR扩增,均获得特异性目的片段;测序及BLAST分析表明HA基因与A/chicken/Viet Nam/Ncvd8/2003(H5N1)的HA基因同源性为98%;NA基因与A/swine/Fujian/1/2003(H5N1)的NA基因同源性为98%,由此该分离株鉴定为H5N1亚型禽流感病毒,按照国际流感病毒系统命名原则将该病毒株暂命名为A/Swan/Guangdong/197/2004。One avian influenza virus strain was isolated from swan in Guangdong in 2004.It was identified with hemagglutination inhibition（HI）,RT-PCR and sequence analysis.These results showed that the isolate had hemagglutination and was inhabited by the standard antibody of H5 avian influenza virus;at the same time,it was amplified by RT-PCR,which was used by the specific identification primers of M,HA genes and NA of avian influenza virus,and gained the specific segments.Sequence analysis demonstrated that HA and NA genes of the isolated strain shared both 98% nucleotide sequence identity to the HA gene of A/chicken/Viet Nam/Ncvd8/2003（H5N1） and the NA gene of A/swine/Fujian/1/2003（H5N1）.Thus,the isolate was identified as a H5N1 subtype avian influenza virus and named A/Swan/Guangdong/197/2004.

关 键 词：禽流感 H5N1亚型 天鹅 分离鉴定 

分 类 号：S852.659.5[农业科学—基础兽医学]