新城疫病毒云南分离株F基因变异特性分析  

Mutation Characteristics of the F Genes of Newcastle Disease Virus From Yunnan Province

作　　者：宋建领[1] 田建国[2] 叶丛华[2] 张文东[3] 赵焕云[3] 吕粤[3] 岳亮[3] 王金萍[1] 邱薇[4] 冯子良[4] 张思东[3] 范泉水[4] 张应国[3] 张富强[4] 

机构地区：[1]云南省热带亚热带动物病毒病重点实验室,云南昆明650224 [2]云南农业大学,云南昆明650223 [3]云南省动物疫病预防控制中心,云南昆明650051 [4]成都军区疾病预防控制中心,云南昆明650032

出　　处：《动物医学进展》2010年第5期14-18,共5页Progress In Veterinary Medicine

基　　金：云南省科技攻关项目(2006NG-24);云南省后备人才基金项目(2009CI061);云南省高层次科技人才培引工程(20080C002);农业部农业公益性行业科研专项(200803026)

摘　　要：采用RT-PCR技术对云南2007年-2009年采集的948份喉气管拭子或组织样品中新城疫病毒F基因进行检测,获得阳性样品65份,选择30份代表性阳性样品采用特异性引物经RT-PCR扩增F基因,纯化后克隆至pMD18-T载体,并对其进行测序。序列比对及系统发育分析结果表明,新城疫病毒云南分离株F基因核苷酸与疫苗LaSota株之间的同源性为70.5%～99.8%,与F48E9株之间的同源性为70.7%～90.8%。系统发育分析表明,30株病毒中21株属于基因型Ⅶ,裂解位点氨基酸为R-R-Q-K-R-F,与传统认为强毒裂解位点氨基酸序列相同;7株属于基因型Ⅱ,5株裂解位点氨基酸为G-R-Q-G-R-L,属于弱毒株裂解位点氨基酸排列特征,2株裂解位点氨基酸为R-R-Q-K-R-F属于强毒裂解位点氨基酸序列;2株病毒与9个基因型的同源性差异较远。The F genes of Newcastle disease virus were detected by the reverse transcriptase-polymerase chain reaction（RT-PCR） from 948 samples which were collected from Yunnan province during 2007 to 2009,65 samples were positive,The F genes were amplified from 30 represented samples by using specific primers,purified and cloned into pMD18-T vector for sequencing.The results of sequence alignment and phylogenetic analysis showed that the nucleotide homologies of F genes among Yunnan province samples with commonly used vaccine strains LaSota were 70.5%-99.8%,the nucleotide homologies with F48E9 strains were 70.7%-90.8%.Phylogenetic analysis showed that the 21 strains of Newcastle disease virus were belonged to genotype Ⅶ,their cleavage site amino acid is R-R-Q-K-R-F,as same as the virulent cleavage site amino acid sequence.7 strains belonged to genotypeⅡ,5 of their cleavage site amino acid were G-R-Q-G-R-L,belonged to the feature of the weak strains;2 of their cleavage site were R-R-Q-K-R-F,belonged to the virulent strains.The homologies between 2 strains and the other 9 genotype strains were highly different.

关 键 词：新城疫病毒 F基因 序列分析 基因型 

分 类 号：S852.659.5[农业科学—基础兽医学]