猪PRRSV云南分离株主要蛋白基因克隆及序列分析  

Cloning and Sequence Analysis of Porcine Reproductive and Respiratory Syndrome Virus Major Protein Genes

作　　者：杨玉艾[1] 肖晶[1] 杨亮宇[1] 初晓辉[1] 毕保良[1] 孙永科[1] 

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201

出　　处：《动物医学进展》2010年第5期18-22,共5页Progress In Veterinary Medicine

基　　金：国家自然科学基金(30960285);云南省教育厅基金(08C0072);云南省科技厅基金(2008CD134;2009CD063);高层次科技人才培引工程基金(2009CI125-13;2009CI125-14)

摘　　要：采用RT-PCR技术,分别对PRRSV云南分离株-YN株编码GP3、GP5、M蛋白的基因进行扩增,获得764、602、524 bp特异性目的片段,经纯化后,克隆至pMD18-T载体中,分别进行序列测定与分析。结果表明,云南PRRSV与已知代表毒株核苷酸序列同源性分别为89.72%～98.80%;遗传进化分析表明,云南分离株与HB-2(sh)/2002、CH-1a等毒株关系最近,与VR-2332、respPRRS mlv等同属一个大分支,而与JXA1、GD、NM1等毒株处于不同的分支。The major protein genes of porcine reproductive and respiratory syndrome virus（PRRSV） YN strains,GP3,GP5 and M,were amplified by RT-PCR,and the special 764bp,602bp and 524 bp fragments were obtained,respectively.The PCR products of GP3,GP5 and M genes were purified and cloned into pMD18-T vector for sequencing and sequence alignment.The results of sequence analysis showed that the homologies of nucleotide of PRRSV genes from Yunnan strains were 89.72%-98.80% with known representative PRRSV strains.The phylogenetic analysis revealed that the YN strain was similar to HB-2（sh）/2002 and CH-1a,etc.YN strain,VR-2332 and respPRRS mlv,etc.belonged to the same group,but the YN strain was not closely related to JXA1,GD,NM1 strains.

关 键 词：猪繁殖与呼吸综合征病毒 基因 克隆 序列分析 

分 类 号：S852.659.6[农业科学—基础兽医学]