一株鸽源新城疫病毒的分离鉴定  

Isolation and Identification of A Newcastle Disease Virus Strain From Pigeon

作　　者：蔡绍鑫[1] 焦培荣[1] 高诗敏[1] 何琴[1] 刘大伟[1] 薛国聪[1] 张桂红[1] 辛朝安[1] 廖明[1] 任涛[1] 

机构地区：[1]华南农业大学兽医学院农业部动物疫病防控重点开放实验室广东省动物源性人兽共患病预防与控制重点实验室,广东广州510642

出　　处：《动物医学进展》2010年第5期23-25,共3页Progress In Veterinary Medicine

基　　金：教育部"长江学者和创新团队发展计划"创新团队项目(IRT0723);公益性行业(农业)科研专项经费项目(200803020-08);广东省科技计划项目(2004B20201030;5500E08020;2007A020100006)

摘　　要：从广东发病鸽群中采集的拭子样本中分离到1株新城疫病毒,用聚合酶链反应(PCR)、血凝抑制试验(HI)及基因测序等对其进行鉴定。结果表明,此分离株具有血凝活性,且可与新城疫病毒标准阳性血清发生特异性抑制反应;用针对新城疫病毒的F基因及P基因特异性鉴定引物对该分离株进行PCR扩增,可扩增出特异性目的片段;测序及BLAST分析表明,其与美国新城疫病毒分离株Pigeon/US(TX)/98的F基因核苷酸序列相似性高达97%以上。毒株被鉴定为鸽源新城疫病毒,并命名为Pigeon/Guangdong/ZQ-17。A Newcastle disease virus（NDV） virus was isolated from pigeon in Guangdong.It was identified with PCR,hemagglutination inhibition and sequence analysis.The results showed the isolate had hemagglutination and was inhabited by the standard antibody of NDV;it was amplified by RT-PCR,which was used by the specific identification primers of F,P of NDV,and gained the specific segments.Blasted and compared with NDV of GenBank in NCBI,the F gene of the isolate shared the closed similarity with the NDV from USA,and the similarity was over 97%.Thus,the isolate was identified as a pigeon origin NDV,named Pigeon/Guangdong/ZQ-17.

关 键 词：新城疫病毒 鸽 分离鉴定 

分 类 号：S852.659.5[农业科学—基础兽医学]