腹泻犊牛病原分离鉴定及药敏试验  

Isolation and Identification of Pathogen from Calf Diarrhoea and Drug Sensitivity Test

作　　者：王建永[1] 张磊[1] 赵月兰[1] 秦建华[2] 

机构地区：[1]河北农业大学中兽医学院,河北定州073000 [2]河北农业大学动物科技学院,河北保定071000

出　　处：《动物医学进展》2010年第5期118-121,共4页Progress In Veterinary Medicine

基　　金：国家"十一五"科技支撑计划项目(2006BAD04A10-4);河北省重大技术创新专项计划项目(07227146Z-4)

摘　　要：从河北省某奶牛场采集腹泻犊牛粪样,进行病原的分离鉴定及耐药性检测。结果表明,分离菌在形态特征、培养特性、生化特性、血清型和致病性等方面与大肠埃希菌基本一致,鉴定为大肠埃希菌。应用大肠埃希菌O抗原定型血清鉴定为O9。肠毒素试验表明,该菌产生热稳定肠毒素。分离菌对多黏菌素、丁胺卡那霉素敏感,对链霉素、红霉素、四环素、庆大霉素和氨苄青霉素则表现出耐药性。Calves＇ lax dejecta was collected and a bacterial strain was isolated from dairy farms in Hebei province.The morphological characteristics,culture characteristics,biochemistry characteristics,serotype,pathogenicity and drug sensitivity of the isolate were detected.The results indicated that these characteristics were the same to Escherichia coli.The isolated strain was identified as O9 by E.coli O-antigen serotypes.The strain E.coli was capable of producing heat-stable enterotoxin（ST） by detection of enterotoxin.The isolated strain was sensitive to some drugs such as polymycin B and amikacin,but resistance to streptomycin,erythromycin,tetracycline,gentamicin,ampicillin.

关 键 词：犊牛 大肠埃希菌 分离鉴定 耐药性 

分 类 号：S853.52[农业科学—临床兽医学] S857.34[农业科学—兽医学]